【作者】 徐加杰； 葛明华； 赏金标； 谭卓； 陈超； 王可敬；

【机构】 浙江省肿瘤医院头颈外科；

【摘要】 目的:探讨PIM-1蛋白参与鼻咽癌细胞转移的机制。方法:采用Lenti-shRNA将CNE-2Z鼻咽癌细胞中的PIM-1的表达进行沉默,并采用Realtime PCR对mir-17-5p的表达进行检测;过表达CNE-2Z鼻咽癌细胞中的mir-17-5p,采用Transwell实验比较mir-17-5p转染前后sh1-PIM-1细胞的迁移能力。结果:Realtime PCR及Western Blot结果显示PIM-1沉默后mir-17-5p的表达水平显著升高。Transwell结果显示,mir-17-5p的过表达增强CNE-2Z细胞的迁移能力。结论:癌基因PIM-1通过调控mir-17-5p的表达,可以调控鼻咽癌肿瘤细胞的转移。更多还原

【Abstract】 Objectives:The purpose of this study was to investigate the role of PIM 1 protein expression in the metastasis of nasopharyngeal carcinoma. Methods:PIM 1 expression in CNE-2Z cell line was knocked down by Lenti-shRNA,the Silence efficiency was evaluated by realtime PCR and Western Blot.The migration of nasopharyngeal carcinoma was analyzed by transwell assay. Results:We found that the expression of mir-17-5p was upregulated after PIM1 knock down.Meanwhile,the migration ability was upregulated after mir-17-5p wastransfected. Conclusion:PIM - 1 can promote the metastasis of nasopharyngeal carcinoma through the regulation of mir- 17-5 p expression regulation.更多还原