【作者】 雷永良； 王晓光； 陈秀英；

【Author】 Lishui Center for Disease Control and Prevention,Lishui,Zhejiang,323000,China LEI Yong-liang WANG Xiao-guang CHEN Xiu-ying

【机构】 丽水市疾病预防控制中心；

【摘要】 目的了解丽水市结核分枝杆菌耐药相关基因rpoB、katG、embB、rpsL和gyrA突变特点。方法采用PCR-DNA直接测序法对47株结核菌分离株和1株标准株5个耐药相关基因主要耐药位点突变进行分析。结果药敏试验共获得耐药结核菌18株,总耐药率为38.309%;序列分析13株存在一个或多个突变位点,未发现碱基的插入或缺失,突变率为27.66/%,二者吻合率72.22%。结论丽水市结核菌单一药物耐药比例较低,耐药性的发生更趋向于耐多种药物。结核菌耐多药性与多个基因突变相关,点突变是本地区结核菌产生耐药的主要形式,部分未发现耐药基因突变位点的耐药结核菌可能还存在其他耐药机制。采集复治结核病人的标本可能是总耐药率偏高的一个重要影响因素。更多还原

【Abstract】 Objective To elucidate the characterization of rpoB,katG,embB,rpsL and gyrA mutation in resistant clinical isolates of Mycobacterium tuberculosis(MTB) from Lishui city.Methods Analyzing mutations of five resistance-related genes in 47 clinical isolates and ltype strain of MTB by PCR-DNA.Results Susceptibility testing were obtained 18 isolates of drug-resistant MTB,38.30%had drug-resistant strains.There are existence one or more mutations in 13 isolates by sequence analysis.No insertion or deletion mutation was found in these specimens,27.66%isolates had mutations located at major locus,both match the rate of 72.22%. Conclusions Single drug-resistant MTB is relatively low in Lishui City,the incidence of resistance tended to be more resistant to multiple drugs.Multidrug-resistance to MTB isolates was related with the mutation of multiple genes.Point mutation is the main modality form of MTB resistant in this region.Some drug-resistant MTB of other resistance mechanisms may need explored.Acquisition retreatment TB specimens may be the high rate of drug resistance is an important factor.更多还原