【作者】 谢作听； 杨慧敏； 周湘静； 俞石芳； 陶志华；

【Author】 XIE Zuo-ting,ZHOU Xiang-jing,YU Shi-fang,TAO Zhi-hua.Department of Blood Transfusion, the First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000

【机构】 温州医学院附属第一医院；

【摘要】 目的调查分析本地区A抗原凝集强度正常且ABO正反定型结果相符的A型与AB型住院患者红细胞表面A1抗原的表达情况。方法采用常规试管法分别对A抗原凝集强度正常且ABO正反定型结果相符的3020例A型和780例AB型住院患者红细胞进行A1抗原筛检,筛检结果阴性的标本采用4℃吸收-56℃放散试验进行A1抗原确认。结果 A型患者与AB型患者筛检试验的A1抗原阳性率分别为99.2%(2995/3020)、95.6%(746/780),阴性率分别为0.8%(25/3020)、4.4%(34/780),组间比较差异有统计学意义(p<0.05);筛检试验阴性标本4℃吸收-56℃放散试验的A1抗原总阳性率为76.3%(45/59),其中A型和AB型患者确认试验的A1抗原阳性率分别为88.0%(22/25)、67.6%(23/34),组间比较差异有统计学意义(p<0.05)。结论 A抗原凝集强度正常且ABO正反定型结果相符并不能完全排除A亚型(或A亚型B),临床有必要对A型和AB型患者常规开展A1抗原检测工作以确保临床输血安全。更多还原

【Abstract】 Objective To investigate and analyze the expression conditions of Al antigen on the red blood cells of group A and group AB patient with abnormal aggulation strengths of A antigen and with consistent results of forward and reverse grouping.Methods Al antigen is screened for 3020 cases of group A and 780 cases of group AB with abnormal aggulation strengths of A antigens and with consistent results of ABO forward and reverse grouping using routine tube test.The negative cases are comfirmed by 4℃absorption and 56℃elution test.Results The positive rate of Al antigens in group A and group AB cases is 99.2%(2995/3020)、95.6%(746/780) in routine tube test respectively.There are significant differences between them(p < 0.05).The total positive rate of Al antigens is 76.3%(45/59) in 4℃absorption and 56℃elution test.Of which,the positive rate of Al antigens in group A cases and in group AB cases is 88.0%(22/25),67.6%(23/34) respectively.There are significant differences between them(p< 0.05).Concusion Group A and group AB patients with abnormal agglutination strengths of A antigens and with consistent results of forward and reverse grouping could not completely excude A subgroup(or AB subgroup).It is necessary that Al antigen be detected for group A and group AB patients routinely to assure clinical blood transfusion safety.更多还原