【作者】 刘敏； 楚生辉； 崔友； 李校堃；

【Author】 LIU Min~1 CHU Sheng-hui~1 CUI You~2 LI Xiao-kun~1 (1.Wenzhou Medical College,Wenzhou 325035,China; 2.Zhejiang Tianrui Pharmaceutical Co.,Ltd.,Ruian 325200,China)

【机构】 温州医学院； 浙江天瑞药业有限公司；

【摘要】 目的利用高效液相色谱建立温郁金挥发油的指纹图谱。方法采用shimadzu VP-ODS C₁₈色谱柱（250mm×4.6mm,5μm）,以乙腈-0.1磷酸溶液梯度洗脱,体积流量为1.0mL/min,柱温30℃,检测波长210nm。结果以=牛儿酮对照,通过对19批浙江不同产地温郁金样品测定,标定了14个共有峰,并通过"中药色谱指纹图谱相似度评价系统软件"和聚类分析等辅助手段,计算相似度等有关参数。结论该方法准确可靠,重现性好,为更好的控制温郁金内在质量提供了科学依据。更多还原

【Abstract】 Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C. wenyujin was established by HPLC with Shimadzu VP-ODS C column(250mm×4.6mm,5μm),acetonitrile-water(0.1 phosp horic acid)gradient elution, the flow rate of 1.0 mL/min,column temperatures of 30°C,and detective wavelength at 210 nm.Results Taking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C.wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software"Similarity Evaluation System for Chromatographic Finger-print of Chinese Materia Medica"published by GPC(Version 2004A) and cluster analysis.Conclusion This method with better accuracy and reproducibility could be used in the quality control of C.wenyujin.更多还原