【作者】 过建春； 包剑锋；

【机构】 杭州市第六人民医院；

【摘要】 目的:探讨TGF-β₁基因多态性与慢乙肝肝纤维化的关系。方法:以肝组织病理诊断为区分慢性HBV感染者纤维化程度的主要方法,用DNA测序法分析173例乙肝病毒感染病例和52例健康体检者的TGF-β₁基因密码子10和密码子25、启动子区-800 G>A和-509 C>T四个位点基因型在不同肝纤维化程度分组中的分布。结果:密码子10基因型分布在不同纤维化程度分组中的差异有显著性统计学意义（P<0.05）,组间分割两两比较,肝硬化组与S1-2组比较有显著性统计学意义（P<0.005）。肝硬化组中密码子10基因型以LeuLeu型为主（47.6%）,非肝硬化组LeuLeu型是23.5%,两组之间差异有非常显著性统计学意义（P<0.01）。其余基因位点在两组间没有统计学意义（P>0.05）。肝硬化组密码子10等位基因Leu频率是63.1%,Pro频率为36.9%,而非肝硬化组密码子10等位基因Leu频率是51.1%,Pro频率为48.9%,两组之间比较具有非常显著性统计学意义（P<0.05）,其余基因位点在两组间没有统计学意义（P>0.05）。结论:TGF-β₁密码子10基因多态性与乙肝肝硬化的发生有关,LeuLeu基因型与肝硬化的遗传易感性有关。更多还原

【Abstract】 Objective:To study the relation between TGF-β1 genic polymorphisms and liver fibrosis induced by chronic HBV infection. Methods:The liver fibrosis grades of 173 cases with chronic HBV infection were diagnosed mainly after liver biopsy.These patients and 52 healthy cases(control group) were analyzed for TGF-β1 polymorphisms using DNA sequence analysis.Results:There was very significant difference for codon10Leu>Pro polymorphism among the groups of different fibrosis grades(P<0.05).And there was significant difference between the liver cirrhosis group and S1-2 group(P<0.005).The frequencies of genotype LeuLeu at codon10 in the liver cirrhosis group and non-cirrhosis group were 51.1%and 23.5%respectively,and the difference was very significance(P<0.01).The frequencies of allele at codon10 in liver cirrhosis group and non-cirrhosis group were allele Leu 63.1%versus 51.1%and allele Pro 36.9%versus 48.9%respectively,and the difference was significant between two group(P<0.05).The frequencies of genotype and allele at codon25、-800 G>A and -509 C>T were no significant difference among different groups(P>0.05).Conclusion:This study suggests that TGF-β1 gene codon10 genic polymorphis might play a important role in the development of liver cirrhosis.The genotype LeuLeu and allele Leu at codon10 in patients with chronic HBV infection might be associated with the hereditary susceptibility to cirrhosis.更多还原