节点文献

骨碎补提取物对骨髓间充质干细胞向成骨细胞分化及CXCL12表达的影响及意义

Effects and significance of drynaria extract on expressions of CXCL12 and osteogenic differentiation of bone marrow stromal cells

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 刘国岩徐展望徐琬梨

【Author】 LIU Guo-yan~1,XU Zhan-wang~1,XU Wan-li~2 (1 Affiliated Hospital,Shandong University of Traditional Chinese Medicine,Jinan 250014,China;2 Shandong University of Traditional Chinese Medicine,Jinan 250355,China )

【机构】 山东中医药大学附属医院山东中医药大学

【摘要】 目的:体外实验研究中药骨碎补对大鼠骨髓间充质干细胞(BMSCs)的增殖及分化,及对趋化因子CXCL12分泌的影响机制。方法:采用中药干预培养细胞的方法,利用骨碎补单味药物与培养基联合培养大鼠BMSCs,通过ALP染色检测其促BMSCs成骨分化能力、RT-PCR检测其促BMSCs分泌趋化因子CXCL12能力。结果:骨碎补药物各组ALP染色检测BMSCs成骨分化能力均明显优于空白组及条件培养组(P<0.05),骨碎补药物各组之间没有显著性差异。骨碎补药物各组RT-PCR检测CXCL12的表达明显优于空白组及条件培养组(P<0.01),0.002g/mL药物组优于0.02g/mL及0.0002g/mL药物组(P<0.05)。结论:中药骨碎补可促进BMSCs成骨分化和增殖,促进BMSCs分泌趋化因子CXCL12。

【Abstract】 Objective:To study the effect of drynaria extract on proliferation and differentiation of rat bone marrow stromal cells(BMSC_S) and the mechanism of promoting the secretion of the chemokine CXCL12 in vitro.Methods:With the method of interference of Chinese medicine cell culture,single drynaria combined with medium co-culture of rat BMSCs,inspect ability of osteogenic differentiation of BMSCs through alkaline phosphatase stain detection and ability of increasing the secretion of CXCL12 through RT-PCR detection.Results:The alkaline phosphatase stain of testing indexes that the drynaria drug groups were superior to blank group and conditional group(.P<0.05),the drynaria drug groups had no obvious differences.The results of CXCL12 expression by RT-PCR detection showed the drynaria drug groups were much better than the blank group and the conditional group(P<0.01),the 0.002g/mL drynaria group was superior to 0.02g/mL and 0.0002g/mL drynaria groups(P<0.05). Conclusion:Traditional Chinese medicine drynaria could promote osteogenic differentiation of BMSCs and increase the secretion of chemokine CXCL12.

【基金】 山东省中医管理局(No.2005-172)~~
  • 【会议录名称】 第四届全国中医药博士生优秀论文专辑
  • 【会议名称】全国中医药博士生学术交流会暨第四届全国中医药博士生优秀论文颁奖会议
  • 【会议时间】2013-03-29
  • 【会议地点】中国上海
  • 【分类号】R285.5
  • 【主办单位】中国中医药学会(China Association of Chinese Medicine)、上海中医药大学、《中华中医药杂志》社
节点文献中: 

本文链接的文献网络图示:

本文的引文网络