【作者】 刘国岩； 徐展望； 徐琬梨；

【Author】 LIU Guo-yan~1,XU Zhan-wang~1,XU Wan-li~2 (1 Affiliated Hospital,Shandong University of Traditional Chinese Medicine,Jinan 250014,China;2 Shandong University of Traditional Chinese Medicine,Jinan 250355,China )

【机构】 山东中医药大学附属医院； 山东中医药大学；

【摘要】 目的:体外实验研究中药骨碎补对大鼠骨髓间充质干细胞(BMSCs)的增殖及分化,及对趋化因子CXCL12分泌的影响机制。方法:采用中药干预培养细胞的方法,利用骨碎补单味药物与培养基联合培养大鼠BMSCs,通过ALP染色检测其促BMSCs成骨分化能力、RT-PCR检测其促BMSCs分泌趋化因子CXCL12能力。结果:骨碎补药物各组ALP染色检测BMSCs成骨分化能力均明显优于空白组及条件培养组(P<0.05),骨碎补药物各组之间没有显著性差异。骨碎补药物各组RT-PCR检测CXCL12的表达明显优于空白组及条件培养组(P<0.01),0.002g/mL药物组优于0.02g/mL及0.0002g/mL药物组(P<0.05)。结论:中药骨碎补可促进BMSCs成骨分化和增殖,促进BMSCs分泌趋化因子CXCL12。更多还原

【Abstract】 Objective:To study the effect of drynaria extract on proliferation and differentiation of rat bone marrow stromal cells(BMSC_S) and the mechanism of promoting the secretion of the chemokine CXCL12 in vitro.Methods:With the method of interference of Chinese medicine cell culture,single drynaria combined with medium co-culture of rat BMSCs,inspect ability of osteogenic differentiation of BMSCs through alkaline phosphatase stain detection and ability of increasing the secretion of CXCL12 through RT-PCR detection.Results:The alkaline phosphatase stain of testing indexes that the drynaria drug groups were superior to blank group and conditional group(.P<0.05),the drynaria drug groups had no obvious differences.The results of CXCL12 expression by RT-PCR detection showed the drynaria drug groups were much better than the blank group and the conditional group(P<0.01),the 0.002g/mL drynaria group was superior to 0.02g/mL and 0.0002g/mL drynaria groups(P<0.05). Conclusion:Traditional Chinese medicine drynaria could promote osteogenic differentiation of BMSCs and increase the secretion of chemokine CXCL12.更多还原