【作者】 刘妙； 刘蕾； 李宇航； 钟相根； 贾旭； 孙燕； 王谦； 王坦； 全贞雪； 马小鑫； 田浩君； 刘晓辉；

【Author】 LIU Miao,LIU Lei,LI Yu-hang,ZHONG Xiang-gen,JIA Xu,SUN Yan,WANG Qian,WANG Tan, QUAN Zhen-xue,MA Xiao-xin,TIAN Hao-jun,LIU Xiao-hui (Beijing University of Chinese Medicine,Beijing 100029,China)

【机构】 北京中医药大学；

【摘要】 目的:探讨不同时间点慢性阻塞性肺疾病(COPD)大鼠血浆、肺组织和结肠组织中生长激素释放肽(ghrelin)表达的变化。方法:采用单纯熏烟法建立COPD大鼠模型,将34只Wistar随机分为正常组、4周模型组和8周模型组,分别记录各组大鼠食量、体质量,用放免法测定大鼠肺、肠及血浆中ghrelin含量。结果:模型组大鼠肺、肠组织病理改变显著;与正常组相比,模型组食量减少,体质量下降;8周模型组血浆ghrelin水平较正常组显著性降低(P<0.05);4周、8周模型组肺组织ghreli n水平与正常组比较显著降低(P<0.05),但两组之间无显著性差异;4周、8周模型组肠组织ghrelin水平与正常组比较显著性升高(P<0.05),但2组之间无显著性差异。结论:通过观察熏烟引起的COPD大鼠的各脏器病理变化、食量、体质量以及ghrelin在肺、肠、血清中的变化可以看出,肺肠之间可能存在着某种互动调节机制。更多还原

【Abstract】 Objective:To investigate the expression of ghrelin of blood plasma,lung tissue and colon in chronic obstructive pulmonary disense(COPD) rats in variety time point.Methods:COPD was simply induced by smoking.Study was performed with 34 rats exposed to cigarette smoke which randomly separated into three groups:control group,4-week cigarette smoke exposed group(4w) and 8-week cigarette smoke exposed group(8w).Dietary intake and body weight were weighted every week.Ghrelin in Blood plasma,lung and large intestine tissue level was measured by radioimmunoassay.Results:Dietary intake and body weight were significantly declined in cigarette smoke exposed group.The level of ghrelin in blood plasma of control group was respectively.There were significant differences between control group and 8-week cigarette smoke exposed group(P<0.05).The level of ghrelin in lung and intestine tissue of control group was respectively.Compared with control group, 4w and 8w group were significant difference(P<0.05,P<0.01),but not between 4w and 8w group.Conclusions:From the perspective of pathological changes in lung and intestine tissue induced by smoking,appetite,body weight,and ghrelin changes in lung,intestine and serum,there existed some interaction regulatory mechanism between lung and intestine.更多还原