【作者】 孟楣； 王晓玉； 姜辉； 刘健； 张雅茗；

【Author】 Meng Mei;Wang Xiaoyu;Jiang Hui;Liu Jian;Zhang Yarning (The 1st Affiliated Hospital of Anhui College of Traditional Chinese Medicine;Grade 3 Laboratory of TCM Preparation of State Administration of TCM;Hefei 230031)

【机构】 安徽中医学院第一附属医院、国家中医药管理局中药制剂三级实验室；

【摘要】 目的 :建立复方芪薏胶囊中水溶性蛋白的特征图谱。方法 :通过SDS-PAGE电泳,分离复方芪薏胶囊中不同分子量的水溶性蛋白,薄层扫描法定量,电泳为1.0mm×25齿试样格、浓缩胶浓度为4.5%、分离胶浓度为12.5%、分离电压100V、上样量5μl、染色2h、脱色4h:扫描波长,入_A=570nm,λ_?=465nm。结果 :复方芪薏胶囊中水溶性蛋白成分含量不均,有7个特征带峰,其中主带2条,分子量为16.38kDa、17.07kDa左右。结论 :该方法可以用于复方芪薏胶囊中水溶性蛋白成分测定及指纹特征图谱构建,具有良好的精密度、稳定性和重复性,为有效控制该制剂质量提供了新方法。更多还原

【Abstract】 【Objective】To establish Feature Maps of water-soluble proteins in Fufang Qiyi Capsules.Method: Water-soluble proteins in Fufang Qiyi Capsules were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and quantified by TLC-Scanning.Electrophoresis and Scanning conditions contained 1.0mmx25 teeth comb,4.5%stacking gel,12.5%separating gel,100V separation voltage,5ul sample injection, staining for 2h,destaining for 4h,λ_S=570nm,λ_R=465nm.Results:.Water-soluble proteins in Fufang Qiyi Capsules with different content have seven characteristic peaks,two main bands with the molecular weight of 16.38 kDa and 17.07 kDa.Conclusion:The Method can be used in determination and establishment Feature Maps of water-soluble proteins in Fufang Qiyi Capsules with favourable stability,repeatability and precision,which provided a new method for the effective control of the quality of Fufang Qiyi Capsules.更多还原