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芪棱汤对大鼠脑缺血再灌注后热休克蛋白70的影响
Influence of Qileng Decoction on expression of Heat shock protein 70 after focal Cerebral Ischemic Reperfusion in Rats
【Author】 Deng shifang~1 Jiang hongyu~1 Zhangshiwei~1 Caoping~2 1.Shenzhen People’s Hospital(shenzhen 518020) 2.Shenzhen longgang central’s Hospital(shenzhen 518000)
【摘要】 目的观察芪棱汤对脑缺血再灌注损伤后热休克蛋白70的影响,为益气养阴活血法治疗缺血性中风提供实验依据。方法将大鼠随机分为假手术组、模型组、补阳还五汤组及芪棱汤组。采用颈内动脉线栓加环扎法复制大鼠脑缺血再灌注模型。于再灌注后不同时间点观察大鼠缺血脑组织热休克蛋白70(Heat shock protein 70,Hsp 70)蛋白表达。结果假手术组无Hsp70蛋白表达,模型组、芪棱汤组Hsp70表达差异有显著性。芪棱汤组Hsp70蛋白表达优于其它各组。结论芪棱汤可以促进Hsp70的蛋白表达,从而对脑缺血再灌注所导致的脑损伤起保护作用。
【Abstract】 Objective:To explore the Influence of Qileng Decoction on expression of Heat shock protein 70 after focal cerebral ischemic reperfusion in Rats,and to provide the scientific foundation for benefit vital energy(BVE),nourishing yin(NY),activating blood circulation(ABC)on treating ischemic stroke.Methods:Therats were randomly divided into four groups including sham-operation,model group,Buyanghuanwu(BYHW) decotion group and Qileng decoction group.The model of focal cerebral ischemic reperfusion injury was made by using thread embolizing and fastening of the ICA in rats.Heat shock protein 70 were observed byimmunohistochemical method at different time point after cerebral ischemic reperfusion.Results; The expression of Heat shock protein 70 was not found in sham-operation.There was statistic significance among model group,Qileng decoction group.and the effect of Qileng decoction group was superior to that of other groups.Conclusion:Qileng decoction plays the neuron protective effects aftercerebral ischemic reperfusion by promoting the expression of the Heat shock protein 70.It may be one of the mechanism of BE-NY-ABC treating ischemic stroke.
【Key words】 cerebral ischemic reperfusion; Qileng decoction; BE-NY-ABC; Heat shock protein 70.;
- 【会议录名称】 中华中医药学会血栓病分会第四次学术研讨会暨广东省中医药学会血栓病专业委员会首届学术研讨会论文集
- 【会议名称】中华中医药学会血栓病分会第四次学术研讨会暨广东省中医药学会血栓病专业委员会首届学术研讨会
- 【会议时间】2010-09-01
- 【会议地点】中国广东深圳
- 【分类号】R285.5
- 【主办单位】中华中医药学会血栓病分会