【作者】 李宁； 唐启盛； 侯秀娟； 赵瑞珍； 黄育玲； 方芳； 臧凤超；

【Author】 LiNing~1,Tang QiSheng~2,HouXiuJuan~3,Zhao RuiZhen~2,Hung Yuling~1 FangFang~4,ZangFengChao~4 (1.Beijing University of Chinese Medicine,Beijing 100029;2.The Third Hospital Affiliated to Beijing University of Chinese Medicine,Beijingl00029;3.DongFang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing100079;4.Molecular Imaging and Functional Imaging Laboratory in Jiangsu Province,NanJing 210009)

【机构】 北京中医药大学； 北京中医药大学第三附属医院； 北京中医药大学东方医院； 江苏省分子影像与功能影像重点实验室；

【摘要】 以广泛性焦虑大鼠为研究载体,探讨其前额叶皮质和海马组织质子磁共振波谱(~1H-MRS)的变化及意义。大鼠分为正常组和模型组各8只,采用慢性情绪应激的方法建立广泛性焦虑大鼠模型,以国际公认的高架十字迷宫试验对其进行评价,在活体状态下,通过pharmascan70/16超导磁共振仪(7.0T)检测双侧海马及前额叶皮质N-乙酰天门冬氨酸(NAA)、胆碱(Cho)、谷氨酸(Glu)、肌酸(Cr)等代谢物水平,分别计算NAA、Cho和Glu与Cr的比值,进而对脑组织代谢进行定性及定量分析。正常组与广泛性焦虑组双侧海马及左侧前额叶皮质代谢物无明显差异(P>0.05),广泛性焦虑大鼠右侧前额叶皮质NAA、Cho相对浓度较正常组显著降低(P<0.05),Glu相对浓度则明显升高(P<0.05)。慢性焦虑情绪的产生可能与右侧前额叶皮质兴奋性神经递质谷氨酸的浓度升高、神经元的损伤或数量减少及细胞内信号转导的异常有关。更多还原

【Abstract】 To investigate the metabolic changes of bilateral prefrontal cortex and hippocampus of rats with generalized anxiety disorder by 7.0T ~1H magnetic resonance spectroscopy(~1H-MRS).Wistar male rats were randomized into normal control and model group(n = 8,per group).Rats with generalized anxiety disorder was established by using chronic emotional stress-induced anxiety method,then researchers observed behavioural changes of model rats and made evaluations by elevated plus-maze test system.1H-MRS was performed to detect the metabolic changes of N-acetylaspartate(NAA),Choline(Cho),Creatine(Cr),Glutamate(Glu) in bilateral prefrontal cortex and hippocampus,and to calculate the ratio of NAA/Cr,Cho/Cr and Glu/Cr.There was no significant difference in bilateral hippocampus and left prefrontal cortex between normal control and model group about metabolic changes(P>0.05).The ratio of NAA/Cr and Cho/Cr in right prefrontal cortex of rats with generalized anxiety disorder were significantly lower than normal control(P<0.05),and the ratio of Glu/Cr were significantly higher than normal control(P<0.05).Chronic anxiety may be related to imbalance of amino acid neurotransmitters,neuronal injury and/or decrease in the number and intracellular signal transduction abnormalitiesin the right prefrontal cortex.更多还原