【作者】 崔亚君； 陈霁昀； 里萍； 韩健； 果德安；

【Author】 Yajun Cui~1,Jiyun Chen~2,Ping Li~2,Jian Han~(2*) (1 School of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine, Shanghai 201203;2 School of Pharmaceutical Sciences,Peking University,Beijing 100083)

【机构】 上海中医药大学中药学院； 北京大学药学院；

【摘要】 目的:研究番荔枝中抗肿瘤活性成分。观察Squamocin对BGC-823和HL-60细胞的影响。方法:对氯仿提取物用正反相柱,HPLC进行系统的成分分离,光谱解析和结构鉴定。用MTT方法检测Squamocin对体外培养的人癌细胞BGC-823和HL-60细胞的细胞毒性作用。结果:从番荔枝Annona squamosa)种子中分离得到9个白色蜡状固体,其结构分别鉴定为Tripoxyrollin（1）,Squamocin（2）,Squamocin-B（3）,Squamocin-G（4）,Moltrilin（5）,Squamocin-J（6）,Squamocin-L（7）,Squamostatin-A（8）,Squamostatin-C（9）,其中Tripoxyrollin是首次从该植物中分离得到的天然产物。在squamocin浓度1x10^-6 and 10μg/ml时,在BGC-823和HL-60细胞第1天就能测到细胞存活率明显降低。结论:本实验从细胞水平证实Squamocin对体外培养的两种人体癌细胞株胃癌（BGC-823）和人急性早幼粒白血病细胞（HL-60）具有很强的杀伤作用,且呈现良好的剂量依赖关系。更多还原

【Abstract】 Objective:To study the cytotoxic compounds from Annona Squamosa.Methods:Using chloroform extraction and silica gel column chromatography to isolate compounds and using spectral means to determine the structures. The cytotoxic activity against human cancer cell lines BGC-823 and HL-60 was assayed by MTT methods.Results:Nine compounds were identified as tripoxyrollin（1）,squamocin（2）,squamocin-B（3）,squamocin-G（4）,moltrilin（5）, squamocin-J（6）,squamocin-L（7）,squamostatin-A（8） and squamostatin-C（9）,respectively.Among them,tripoxyrollin（1） was firstly isolated from Annona squamosa.When BGC-823 and HL-60 cells was treated with squamocin 1×10^-6 and 10g/ml for 1 day,respectively,cells survival ability was remarkably inhibited.Conclusion:Squamocin exhibited potent cytotoxic activity in vitro against two human tumor cell lines（BGC-823 and HL-60 cells）.更多还原