【作者】 贺福元； 邓凯文； 石继连； 刘文龙； 刘平安； 周宏灏；

【机构】 中南大学临床药理研究所； 湖南中医药大学药学院； 湖南中医药大学第一附属医院；

【摘要】 目的:在建立谱效动学总量统计矩理论基础上,用鱼腥草注射剂验证该法。方法:用二维特征变量曲线统计矩原理阐明、并建立中药多成分药效动力学总量参数分析方法,用指纹图谱表达。结果:用该法求得鱼腥草注射剂的十三成分的平均谱效动学参数为:AE_T为457.1%·min·mL^-1、MET_T为42.98h、VET_T为443.0 h²、MCET_T为27.22min、VCET_T为844.4min2,说明鱼腥草注射剂十三成分的平均效应半衰期为29.79h,在0～64.01h内95%药效成分被小鼠体内代谢完成;其中主要成分在GC/MC指纹图谱上的中心出峰时间为27.22min,0～56.28min为95%效应成分出峰时间;全方指纹图谱的谱效动学参数为:AE_T为660.2%min·mL^-1、MET_T为45.98h、VET_T为706.0 h²、MCET_T为22.50min、VCET_T为168.8min²,说明鱼腥草注射剂全成分的平均效应半衰期为31.84h,在0～72.55h内95%药效成分被小鼠体内代谢完成;其中主要成分在GC/MC指纹图谱上的中心出峰时间为22.50min,0～35.49min为95%效应成分出峰时间。结论:中药多成分体系可用指纹图谱表达;能用总量统矩参数能表征中药多成分药效动力学的整体行为,与指纹图谱构建谱效动学数学模型及参数研究体系。谱效动学参数由药物的药效学参数与药动学参数共同决定。更多还原

【Abstract】 AIM to eluciate and establish a new pharmacodynamics mathematical models and parameter analyses,as chromatokinetics,for the Chinese Matria Medica formula（CMMF） with fingerprint chromatography.METHODS The models were been set up by statistic moment principle for the curve of 2-dimensional characteristic variates（C2DCV） in order to establish a new method for chromatographic pharmacodynamics（chromatodynamics） study of the TCMM determined by fingerprint chromatographic,by which the Herba Houttuyniae Injection was used to validate the models.RESULTS By these models and methods the whole pharmacodynamtic parameters of the 13 metabolites in vitro mice administrated by Herba Houttuyniae Injection have been obtained as AE_T 457.1%··min·h·mL^-1;MET_T42.98h;VET_T 443.0 h²;MCET_T 27.22min;VCET_T 844.4 min² that suggested for the 13 metabolites the average half time was 29.79 h and 95%of them were token out within 0～64.01 h,correspondingly the average retention time for GC/MC chromatographic fingerprint was 27.22min and 95%of them retention time were within 0～56.28min,while for all over chromatographic fingerprint,the Chromatodynamic parameters were AE_T as 660.2%··min·h·mL^-1,MET_T45.98 h,VET_T706.0 h²,MCET_T22.50 min,VCET_T 168.8 min² respectively that suggested for the all metabolites the average half time was 31.84 h and 95%of them were token out within 0～72.55 h,correspondingly the average retention time for GC/MC chromatographic fingerprint was 22.50 min and 95%of them retention time were within 0～35.49 min.CONCLSION The total quantum statistic moment can have been utilized to establish the pharmacodynamic models and parameter analyses of the CMMF for multiple components,expressed by fingerprint chromatograph.The Chromatodynamic parameters consisted of pharmacodynamic and pharmacokinetic parameters.更多还原