【作者】 贺福元； 邓凯文； 石继连； 刘文龙； 刘平安； 周宏灏；

【机构】 中南大学临床药理研究所； 湖南中医药大学药学院； 湖南中医药大学第一附属医院；

【摘要】 目的建立用指纹图谱表达的中药多成分药效动学:谱效动学总量统计矩理论与实验研究方法。方法用二维特征变量曲线统计矩原理阐明并建立用指纹图谱表达的中药多成分药效动力学总量参数分析方法,用鱼腥草注射剂验证该法。结果建立了包括零阶矩(AE_T)、药物动力学总量一阶矩(MET_T)、指纹图谱总量一阶矩(MCET_T)、药物动力学总量二阶矩(VET_T)、指纹图谱总量二阶矩(VCET_T)的用指纹图谱表达的中药药效动力学统计矩数学模型及参数体系,建立了用参数计算法、梯形法求算多成分药物总量统计矩模型及参数,沟通了单个成分药效动力学参数与成分总量药效动力学参数的关系。结论谱效动学参数由药物的药效学参数与药动学参数共同决定。更多还原

【Abstract】 AIM to eluciate and establish a new pharmacodynamics mathematical models and parameter analyses,as chromatokinetics,for the Chinese Matria Medica formula（CMMF） with fingerprint chromatography.METHODS The models were been set up by statistic moment principle for the curve of 2-dimensional characteristic variates（C2DCV） in order to establish a new method for chromatographic pharmacodynamics（chromatodynamics） study of the TCMM determined by fingerprint chromatographic,by which the Herba Houttuyniae Injection was used to validate the models.RESULTS It have been established the mathematical expressions for the C2DCV system that consists of six mainly chromatokinetic parameters:①total quantum zero moment as AE_T,Accumulation effects of the C2DCV;②Accumulation effects for total quantum of chromatographic fingerprint per weight as AEPW_T of the C2DCV;③mean effect time for whole C2DCV as MET_T, mean pharmacodynamic time of whole C2DCV;④mean chromatographic effect time of whole C2DCV as MCET_T, expressed by（?）_T;⑤variance of effect time of whole C2DCV as VET_T;variance of mean pharmacodynamic time of whole C2DCV;⑥variance of chromatographic effect time of total quantum as VCET_T,expressed by（?）,and the models or methods to determine and calculate these parameters,by which the whole parameters were bridged to the single component parameters.By these models and methods the whole pharmacodynamtic parameters of the 13 metabolites in vitro mice administrated by Herba Houttuyniae Injection have been obtained as AE_T 457.1%··min·h·mL^-1;MET_T42.98h;VET_T443.0 h²; MCET_T27.22min;VCET_T 844.4 min² that suggested for the 13 metabolites the average half time was 29.79 h and 95%of them were token out within 0～64.01 h,correspondingly the average retention time for GC/MC chromatographic fingerprint was 27.22min and 95%of them retention time were within 0～56.28min,while for all over chromatographic fingerprint,the Chromatodynamic parameters were AE_T as 660.2%··min·h·mL^-1,MET_T45.98 h,VET_T706.0 h²,MCET_T22.50 min,VCET_T 168.8 min² respectively that suggested for the all metabolites the average half time was 31.84 h and 95%of them were token out within 0～72.55 h,correspondingly the average retention time for GC/MC chromatographic fingerprint was 22.50 min and 95%of them retention time were within 0～35.49 min.CONCLSION The total quantum statistic moment can have been utilized to establish the pharmacodynamic models and parameter analyses of the CMMF for multiple components,expressed by fingerprint chromatograph.The Chromatodynamic parameters consisted of pharmacodynamic and pharmacokinetic parameters.更多还原