【作者】 钟振国； 梁红； 钟益宁； 李曼莉；

【Author】 Zhong Zhen-guo~1,Liang hong~2,ZhongYi-ning~1 Li Man-li~1 (1.Center of Research & Development of New Drugs,Guangxi Traditional Chinese Medical University, Nanning 530001,Guangxi Zhuang Autonomous Region,China;2.Guangxi Pharmaceutical school, Nanning Guangxi 530023)

【机构】 广西中医学院； 广西药科学校；

【摘要】 目的:探讨从余甘子叶分离出的抗肿瘤活性成分没食子酸和没食子酸乙酯的抗肿瘤作用。方法:将余甘子叶分别用不同浓度的乙醇进行渗漉提取后,再分别用石油醚、氯仿、乙酸乙酯进行部位分离,对有效部位进行分离纯化,得到没食子酸和没食子酸乙酯;以MTT法和细胞集落形成法观察没食子酸和没食子酸乙酯对人肝癌细胞株Bele7404、人胃癌细胞株SGC7901、小鼠肝癌细胞株H22和小鼠肉瘤细胞株S180四种肿瘤细胞的体外抑制作用。结果:MTT法和细胞集落形成法测定的结果均显示,当没食子酸和没食子酸乙酯浓度在2.5μg/ml以上时,四种肿瘤细胞株的生长均受到明显抑制。结论:没食子酸和没食子酸乙酯在体外对多种肿瘤细胞增殖有明显的抑制作用更多还原

【Abstract】 Objective:To isolate the anti-tumor activity components for Gallic Acid and Gallic ethyl from the 50%ethanol extract of the ethyl acetate fractions of the leaves of Phyllanthus emblica L.Method: The leaves of Phyllanthus emblica L.were were isolated.by column chromatography on silica gel,ethyl acetate parts purification,crystallization of pure crystalline Gallic Acid and Gallic ethyl;The growth inhibition was analyzed by MTT and Cell colony measurement technique in the hepatocellular carcinoma Bele7404,stomach carcinoma SGC7901,hepatoma H22 and fibrosarcoma S180 cells line in vitro.Result: MTT assay and cell colony measurement technique were indicated,the growth of four tumor cells were obviously inhibited when the Gallic acid and Gallic ethyl concentration at 2.5μg/ml and above. Conclusion:The results suggest that Gallic acid and Gallic ethyl were cytotoxic to the variety of the cancer cells.更多还原