【作者】 乔晋琳； 汲广成； 李金牛； 郭长青； 陈裔英； 路平； 付本升； 向东东； 马广昊； 刘灿坤；

【Author】 Qiao Jin-lin,Ji Guang-cheng,Li Jin-niu,Guo Chang-qing,Chen Yi-ying,Lu Ping, Fu Ben-sheng,Xiang Dong-dong,Ma Guang-hao,Liu Chan-kun

【机构】 北京海军总医院康复理疗科； 贵阳中医学院针灸推拿系； 北京中医药大学针灸学院；

【摘要】 目的观察针刀疗法对第三腰椎横突综合征模型大鼠组织中成纤维细胞增殖的影响。方法68只SD大鼠按照随机数字表法分为4组,每组17只。针刀干预组:于造模后15 d,在麻醉下,用针刀于大鼠第三腰椎横突后半段表皮投影处刺入至深筋膜下,行疏通切割两三刀,出针,按压针孔止血;封闭对照组:在无菌条件下,用0.45mm×12mm一次性无菌注射针刺入腰3横突后半段深筋膜下,注入20 g/L利多卡因注射液0.05 mL+地塞米松注射液0.01 mL+维生素B₁₂0.01 mL+氯化钠注射液0.13 mL,出针,按压止血。模型对照组、正常对照组不给予任何干预措施。结果68只SD大鼠进入结果分析。①造模后15 d正常对照组结构正常;其他3组血管充血,并见淋巴小结形成。造模后21 d针刀干预组与封闭对照组大鼠组织内成纤维细胞较少,毛细血管增生扩张,胶原重建开始;模型对照组成纤维细胞较多,胶原分泌增多。造模后30 d模型对照组成纤维细胞增生,可见红细胞散在分布,有增生的毛细血管;针刀干预组与封闭对照组,可见纤维组织排列成行;针刀干预组较其他各组胶原纤维形成减少,瘢痕面积小。②造模后21 d正常对照组可偶见增殖的成纤维细胞;封闭对照组与针刀干预组大鼠组织内的成纤维细胞增殖较少;而此时模型对照组可见成纤维细胞增殖较重,胶原分泌增多。造模后30 d正常对照组可少见增殖的成纤维细胞;模型对照组、封闭对照组、针刀干预组成纤维细胞增殖程度均有增强的趋势,但针刀干预组的增殖程度最低。③造模后21d和30d,针刀干预组的增殖指数与模型对照组相比,差异有非常显著性意义（P<0.01）;封闭对照组细胞的增殖指数与模型对照组在30d时有显著差异（P<0.05）。结论针刀干预对第三腰椎横突综合征模型大鼠的组织修复可能是通过抑制成纤维细胞的增殖水平,从而防止了组织瘢痕挛缩的形成。更多还原

【Abstract】 Objective:To observe the effect of acupotomology intervened third lumbar processus transverses syndrome rat on levels of the fibroblast proliferation. Methods:Sixty-eight rats were divided into 4 groups with the random number table,with 17 rats in each group.Acupotomology intervention group:after 15 days,under anesthesia, with acupotomology in the third lumbar transverse process in rats after the second half of epidermal projection department piercing deep fascia,cut 2 or 3 knives,and compressed hemostasis pinhole.The blockade control group:under sterile condition,0.45 mm×12 mm disposable sterile injection needle was injected into in the third lumbar transverse process in rats after the second half of deep fascia,and 20 g/L lidocaine injection 0.05 mL plus dexamethasone injection 0.01 mL plus Vitamin B12 0.01 mL plus sodium chloride injection 0.13 mL was injected.There were no other intervention in the model control group and normal control group. Results:A total of 68 rats were included in the final analysis.,①After 15 days,the structure of the normal control group was normal;the vascular of the other 3 groups was hyperemic,and the formation of lymphoid nodules could be seen.After 21 days,the fibroblasts were less,capillary was hyperemic and expansive,with the collagen began to reconstruct between the acupotomology intervention group and the blockade control group. There were more fibroblasts,the collagen increased in model control group.After 30 days, the fibroblasts of model control group were hyperemic,scattered red blood cells could be found,and capillaries were proliferated.Fibrous tissue arranged in rows in the acupotomology intervention group and the blockade control group.Compared with other group, there was less collagen fibers with small size of scar than other groups.②After 21 days, the normal control group may be occasional fibroblast proliferation;the fibroblast proliferation of the acupotomology intervention group and the blockade control group were less,but the model group was more.After 30 days,the normal control group can be seen the proliferation of fibroblasts.The trend of fibroblast proliferation has enhanced in model control group,blockade control group and acupotomology intervention group,but the acupotomology intervention group was the least.③After 21 days and 30 days,the proliferation index of acupotomology intervention group and model control group had significant differences(P<0.01),there were significant differences between the blockade control group and the model control group on proliferation index in 30 days(P<.05). Conclusion:The acupotomology intervene the tissue repair of third lumbar transverse vertebrarum syndrome by controlling the proliferation of fibroblasts,which prevent the organization of scar formation.更多还原