【作者】 图雅； 孙素琴；

【Author】 Tuya~(1,2) Sunsuqin~1 1.(Analysis Center of Qinghua University,Beijing,100084,China) 2.(Inner Mongolia University for Nationalities,Tongliao,028000,China)

【机构】 清华大学分析中心； 内蒙古民族大学；

【摘要】 目的:建立蒙药那如3味丸中药效组分的含量测定方法。方法:采用高效液相色谱法测定新乌头碱的含量。色谱柱为Extend-C18(250mm×4.6mm,5μm),流动相为甲醇-0.3mol/L三乙胺(65:35),流速为0.8ml/min,检测波长为235nm,柱温为35℃。结果:经过方法学考察,新乌头碱含量测定方法具有一定的专属性、准确性、重现性和可行性,样品在0.35～1.75μg范围内呈良好的线性关系,平均加样回收率为99.96%,RSD为0.87%。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish a method for determining the content of the mesaconitine in Naru-sanwei Pill.METHOD:HPLC was adopted to determine the content of mesaconitine on Extend-C 18(250mm×4.6mm,5μm) column with methanol-0.3mol/L triethylamine(65:35) as mobile phase,the velocity of flow was 0.8ml/min,the detection wavelength was 235nm,column temperature was 35℃.RESULTS: The linear range of mesaconitme was 0.35～1.75μg and the average recycle rate of the added sample was 99.96%, RSD was 0.87%.CONCLUSION:The method is convenient and accurate with satisfactory separation effect, wide linear range,and high sensitivity,and available for quality control of this leechdom.更多还原