【作者】 郭长青； 胡波； 孙红梅； 吴海霞； 乔晋林； 许红； 李晓泓； 刘乃刚； 卢婧； 刘琳； 谷世喆；

【Author】 GUO Chang-qing~1 HU Bo~1,,SUN Hong-mei~2,WU Hai-xia~2,Qiao jinlin~3 XU Hong~2,LI Xiao-hong~1,LIU Nai-gang~1,LU Jing~1,LIU Lin~1,GU Shi-zhe~1 (1College of Acu-moxibustion,2 Department of Anatomy,Beijing University of Chinese Medicine, Beijing 100029)

【机构】 北京中医药大学针灸学院； 北京中医药大学解剖教研室； 海军总医院；

【摘要】 目的:观察第三腰椎横突综合征模型大鼠局部组织相关细胞因子的含量变化,探讨针刀松解法治疗机制。方法:选用SD大鼠28只,随机分为正常组、模型组、电针组、针刀组,后三组首先用一侧第三腰椎横突尖处放置明胶海绵的方法建立第三腰椎横突综合征的动物模型,14天后,后两组分别给予6次电针和2次针刀松解干预治疗。造模56天后,取大鼠第三腰椎横突周围局部组织,采用放射免疫法检测局部组织IL-6、IL-10、TNF-α、TGF-β含量。结果:模型组大鼠IL-6、IL-10、TNF-α、TGF-β含量都明显升高(P<0.01);针刀组IL-6、IL-10、TNF-α、TGF-β含量较模型组降低,电针组IL-6、IL-10含量降低(P<0.01,P<0.05)。结论:针刀松解法可以减轻局部组织内炎性细胞因子的产生,这可能是针刀治疗软组织损伤、改善局部组织瘢痕粘连状况疗效更优的原因之一。更多还原

【Abstract】 Objective:To observe the change of cytokine in local tissue,and tostudy the mechanism of acupotomy dissolution in rats with transverse syndrome of the third lumbar vertebrae.Methods: Twenty-eight SD rats were randomly divided into normal control group,model group,acupotomy dissolution group and electroacupunture(EA) group,with 7 cases in each group.The model in rats with the third lumber vertebrae transverse process syndrome was established by inserting gelfoam into the deep subfascial part of the 3rd lumbar transverse process.14 days later,Acupotomy dissolution group and EA group are used EA therapy 6 times and lysis of acupotomology 2 times respectively.Scars and adhesion and L-6、IL-10、TNF-αTGF-βcontents in local tissue were detected with radioimmunoassay at 56 day,. Results:In model group,L-6、IL-10、TNF-α、TGF-βcontents were increased significantly(P<0.01), inflammatory reaction was intense,the scars and adhesion were formed obviously.In acupotomy dissolution group,L-6、IL-10、TNF-α、TGF-βcontents were decreased significantly(P<0.01,P<0.05).In EA group,L-6、IL-10 contents were decreased too(P<0.01,P<0.05).Inflammatory reaction,the scars and adhesion of the two groups were not obvious.Conclusion:Acupotomy dissolution can adjust the inflammatory cytokine,reduce the damage of inflammatory reaction to local tissue.This may be the reason why acupotomy dissolution was more effective on reducing the scars and adhesion in local tissue.更多还原