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补肾调经方对ASR卵巢颗粒细胞凋亡及相关酶Caspase-3的影响
The Effect of Bushentiaojing Precipe on the expression of ovarian granular apoptosis and caspase -3 in ASR
【作者】 马惠荣; 杜惠兰; 宋翠淼; 段彦苍; 杨秀芳; 李国明;
【Author】 Ma Hui-rong,Du Hui-lan,Song Cui-miao,et al.
【机构】 河北医科大学中西医结合学院; 河北医科大学基础课部;
【摘要】 目的:探讨补肾调经方对ASR卵巢颗粒细胞凋亡及相关酶Caspase-3的影响。方法:利用免疫组化法,检测并比较ASR模型组、正常组、补肾调经方高剂量组和低剂量组大鼠卵巢颗粒细胞凋亡及相关酶Caspase-3的表达。结果:免疫组化结果显示,各组卵巢颗粒细胞凋亡及相关酶Caspase-3的平均灰度大小排列顺序为:模型组<补肾低剂量组<补肾高剂量组<正常组。结论:补肾调经方可不同程度地减少颗粒细胞Caspase-3和凋亡的表达。
【Abstract】 Objective:The purpose of this study was explore the effect of Bushentiaojing Precipe on the expression of ovarian granular apoptosis and caspase - 3 in ASR.Methods:Used the method of Immunocytochemistry to observe and compare the expression of granular apoptosis and caspase - 3 in ASR,normal,high - dose and low - dose group.Results:There were stronger expression of granular apoptosis and caspase - 3 in ASR than that in other 3 groups,weaker expression in treated rats,weakest expression in normal group.Conclusions:Bushentiaojing Precipe can decreased the expression of granular apoptosis and caspase - 3 in ASR.
【Key words】 Bushentiaojing Precipe; ASR; ovary; granular apoptosis; Caspase - 3;
- 【会议录名称】 全国第八次中医妇科学术研讨会论文汇编
- 【会议名称】全国第八次中医妇科学术研讨会
- 【会议时间】2008-10-01
- 【会议地点】中国四川成都
- 【分类号】R285.5
- 【主办单位】中华中医药学会(China Association of Chinese Medicine)