【作者】 沈霖； 郭向飞； 武嘉林； 帅波；

【机构】 华中科技大学同济医学院附属协和医院； 新疆华世丹药业股份有限公司；

【摘要】 目的:研究阿胶强骨口服液(donkey-hide glue reinforcing bone oral solution,DGRBOS)对SD大鼠血液25-羟基维生素D3(25-OH-VD3)、1,25-羟基维生素D3(1,25-(OH)2-VD3)和肝脏VDR基因表达的影响,探讨DGRBOS治疗骨质疏松的疗效机制。方法:6月龄SD大鼠36只,随机分为A组(假手术组),B组(卵巢切除+生理盐水组),C组(卵巢切除+阿胶强骨口服液组),每组12只。6个月后取材检测。采用ELISA法对去势大鼠血清25-OH-VD3和1,25-(OH)2-VD3进行研究,用荧光定量PCR对肝脏VDR基因进行定量分析。结果:C组(卵巢切除+阿胶强骨口服液组)血液中25-羟基维他命D3浓度和1,25-羟基维他命D3浓度与B组(卵巢切除+生理盐水组)比较明显增高,其中对25-羟基维他命D3浓度增高尤为明显,已接近A组(假手术组),p=0.002(p<0.01),差异有明显的统计学意义。荧光定量PCR(FQ-PCR)结果B组与C组相比,p=0.004(p<0.01),说明扩增效率的差异有明显的统计学意义。结论:阿胶强骨口服液可以增加去势大鼠血液中25-OH-VD3、1,25-(OH)2-VD3的浓度,同时上调肝脏中VDR的表达水平,这是阿胶强骨口服液治疗骨质疏松的机制之一。更多还原

【Abstract】 Objective:To investigate the effects of donkey-hide glue reinforcing bone oral solution（DGRBOS） on expression of 25-OH-VD₃、1,25-（OH）2-VD（?） and VDR in rats,and explore the mechanism of treating osteoporosis with DGRBOS.Methods::12 rats were randomly selected from 36 female SD rat s aged 6 months which were equally divided into group A of sham operation with normal physiological saline.The left 24 rat s were randomly divided into 2 groups after ovariectomy:B（group of osteoporotic animal model with normal physiological saline）,C（treated group of osteoporotic animal model with DGRBOS）.Each group included 12 rats.The concentration of 25-OH-VD₃、1,25-（OH）2-VD（?） were detected by ELISA,the expression of VDR mRNA in liver tissue was detected by real time fluorescence quantitative PCR（FQ - PCR） method.Results:The concentration of 25-OH-VD₃、1,25-（OH）)2_VD（?） in C group was higher than B（P<0.01）,which was closed to group of A（sham operation）.the expression of VDR mRNA on gene level in liver tissue of C group was higher than B（P<0.01） Conclusion:DGRBOS can not only improve the concentration of 25-OH-VD₃、1,25-（OH）（?）-VD（?）,but also increasing regulate the VDR mRNA.更多还原