【作者】 毕葳； 雷海民； 李强； 龚卫红； 黄娴； 周玉新；

【Author】 BI wei,LEI haimin,LI qiang,ZHOU yuxin,HUANG xian,GONG wei-hong (Beijing University of Traditional Chinese Medicine,Beijing 100102,China)

【机构】 北京中医药大学中药学院；

【摘要】 目的:应用Rp-HPLC对不同产地虎杖药材进行指纹图谱研究。方法:Kromasil C₁₈色谱柱（5μm,4.6×150mm,北京分析仪器厂,SN:22209）乙腈-水二元梯度洗脱,流速1ml/min,检测波长303nm。结果:根据选择的色谱条件制订了不同产地虎杖药材的指纹图谱。结论:色谱图对各产地虎杖药材中各成分得到了很好的分离,相似度0.9,可作为虎杖药材的专属性指纹图谱。更多还原

【Abstract】 Objective:a fingerprint of raw material of Rhizoma polygoni cuspidati has been studied according to 10 sources.Method:Kromasil C18 column was used,with mixtures of acetonitrile and water as mobile phase in a gradient mode.The wavelength of measurement was 303nm.Result:According to the selected chromatographic conditions,a good fingerprint of Rhizoma polygoni cuspidati has been described.Conclusion:The method is simple,accurate with good reproducibility.It may be practical value for the quality control of sample for Rhizoma polygoni cuspidati.更多还原