【作者】 冯梅； 陈云波； 王奇； 程淑意； 梁伟雄； 温泽淮；

【Author】 FENG Mei,CHEN Yun-bo,WANG Qi,et al (Guangzhou University of TCM,Guangzhou 510405,China)

【机构】 广州中医药大学；

【摘要】 [目的]探讨人参皂苷Rg1对β淀粉样蛋白_25-35(Aβ_25-35)诱导的SD大鼠星形胶质细胞核因子-κB（NF-κB）的活化及其对炎性因子白细胞介素-6（IL-6）的影响。[方法]用人参皂苷Rg1预处理星形胶质细胞24 h（终浓度分别为0、2、4、8、16μmol/L）,然后再用Aβ_25-35和人参皂苷Rg1对星形胶质细胞共同孵育24 h,四甲基偶氮唑盐（MTT）检测各组的细胞活性;用激光扫描共聚焦显微镜（LSCM）检测分析各组的细胞浆与细胞核内NF-κB的激活程度;用酶联免疫吸附试验（ELISA）的方法检测IL-6的蛋白含量。[结果]人参皂苷Rg1各组的细胞活性均明显高于模型组（P<0.05）,并且能下调NF-κB的激活水平,减少IL-6的分泌（P<0.05）,该作用以2、4μmol/L为显著。[结论]人参皂苷Rg1对Aβ_25-35诱导星形胶质细胞模型的神经保护作用可能是通过阻断NF-κB的活化,减弱炎症反应,从而提高了细胞的生存率,且此作用以低浓度为好。更多还原

【Abstract】 [Objective]To explore the effect of panaxsaponin Rg1 on nuclear factorκB activation and inflammation reaction of SD rats astrocyte induced by Aβ_25-35.[Methods]To carry out pretreatment astrocyte for twenty-four hours by using panaxsaponin Rg1 and incubate in common astrocyte for twenty-four hours by using panaxsaponin Rgl and Aβ_25-35.To determine cell activity of every group by MTT method,analysze activation extent of cytoplasm and intranuclear NF-κB by LSCM method,determine IL-6 protein content by ELISA method.[Result]The cell activity of panaxsaponin Rg1 group was obviously high than that of model group（P<0.05） and panaxsaponin Rg1 may down regulate activation level of NF-κB and reduce IL-6 level（P<0.05）.[Conclusion]The panaxsaponin Rg1 had nerves protective effect on astrocyte induced by Aβ_25-35. The effcts may be relationship with intercepting NF-κB activation,weakening inflammatory reaction and enhancing cellular survival rate and it had best effect on low density group.更多还原