【作者】 杨译； 姜辉； 洪锴； 白泉； 唐文豪； 赵连明； 刘德风； 毛加明； 袁人培； 陈茜； 李斌； 魏南；

【Author】 Yang yi,Jiang hui,Hong kai,Bai quan,Tang wenhao,Zhao lianming,Liu defeng,Mao jiaming Yuan renpei,Chen qian,Li bin,Wei nan Department of Urology,the Third hospital of Peking University,Beijing100191,China

【机构】 北京大学第三医院泌尿外科；

【摘要】 目的:分析男性不育患者年龄与精子DNA碎片率和精液常规参数的相关性。方法:按照年龄将85例男性不育患者分为三组。20～29岁组(n=31),30～35岁组(n=28),36～50组(n=26),采用染色质扩散(SCD)实验检测精子DNA碎片率,按照世界卫生组织标准检测精液常规各参数。结果:年龄与精子活力存在负相关关系(r=-2.31,P<0.05),但与精子密度,精子形态和精子DNA碎片无相关性。精子密度与精子形态存在正相关关系(r==0.528,P<0.01),与精子活力存在正相关关系,(r=0.3 1,P<0.01)。精子DNA碎片率与精液常规各参数无相关性。结论:随着男性年龄的增长,精子的活力可能降低,并影响男性的生育力,精子DFI与精液常规各参数无相关性。故精子DNA碎片检测可做作为精液常规分析的补充,进一步反映男性的生育能力。更多还原

【Abstract】 Objective:To analyze the correlation of age and sperm DNA fragmentation with semen parameters in infertile males. Methods:85 cases of infertile males were divided by age into three groups.20～29 age group(n = 31),30～35 age group(n = 28),36 ~ 50 group,(n = 26),sperm DNA fragmentation was measured by sperm chromatin dispersion(SCD).Semen conventional analysis was performed based on WHO criteria(1999). Results:Age negatively correlated with sperm motility(r = -2.31,P <0.05),but is independent of sperm fragmentation,sperm concentration and sperm morphology.Sperm concentration was found Highly statistically significant positive correlated with sperm morphology(r=0.528,P< 0.01) and sperm motility(r=0.31,P< 0.01).No correlations between sperm DNA fragmentation and semen parameters were found. Conclusion:increased age in infertile men is associated with a decline in sperm motility and would exert a negative influence on male fertility.Sperm parameters and DNA damage are complementary,rather than linked.Measurement of sperm DNA fragmentation would be useful for the evaluation of male fertility更多还原