【作者】 王怀秀； 李弘； 袁彩霞； 曹莹丽； 秦琴；

【Author】 Wang Huaixiu,Li Hong,Yuan Caixia,Cao Yingli,Qin Qin Reproductive Medicine Unit of Shanxi Provincial Peoples’Hospital(Taiyuan 030012)

【机构】 山西省人民医院生殖医学科；

【摘要】 目的探讨在体外培养条件下生精细胞向单倍体精子细胞分化以及精子细胞变形为精子的可能性。方法选择9例无精子症患者进行睾丸活检,分离生精小管细胞后在终浓度为50 U/L卵泡刺激素(FSH)和1μmol/1睾酮(T)的改良人输卵管液(HTF)培养液中进行体外培养,所有标本均在培养前及培养后24小时对精子及其它细胞进行计数,计算各种细胞在细胞总数中的比例;并用流式细胞仪对其中两例患者培养前和培养后24小时的细胞进行细胞倍体分析。结果与培养前相比,培养后精子比例显著增加(p=0.004)。细胞倍体分析显示,培养前可见4n、2n和1n波峰,培养后4n波峰消失,1n波峰显著增宽和增高。结论生精细胞体外培养可使生精细胞发生减数分裂,并使精子细胞向精子变形。更多还原

【Abstract】 Objective To investigate the possibility of differentiation and spermiogenesis of spermatocytes under in vitro condition.Methods Cells were prepared from seminiferous tubules from 9 azoospermia patients after testis biopsy and cultured in medium containing follicle stimulating hormone(FSH) 50U/L and testosterone(T)1μmol/l.Sperms and cells of other types were counted and the proportion of every cell type was calculated before and 24 hours after culture.Flow cytometry was conducted before and 24 hours after culture in 2 cases to analyse the ploidy of the cells.Results Sperm proportion was significantly increased after culture(p=0.004). Flow cytometry demonstrated that the diagram of 4n、2n and In converted to 2n and broader 1n. Conclusion Meiosis of spermatocytes and the transformation of spermatid into sperm could arise in in vitro culture.更多还原