Based on Quantum dots detection and immuno-fluorescence imaging of anti-nuclear antibody in different tissue cells

【作者】 虞伟； 李晓军； 张宇； 罗冰； 房国祥； 白彩琴； 武建国； 顾宁；

【Author】 Wei YU Xiao-jun LI Yu ZhANG Bing LUO Guo-xiang FANG Cai-qin BAI Jian-guo WU Ning GU 1.Research Institute of Clinical Laboratory Medicine,PLA.Nanjing General Hospital of Nanjing Command,210002,China. 2.Key Laboratory of Biomaterials and devices of Jiangsu Province,Southeast University,Nanjing 210096,China

【机构】 Research Institute of Clinical Laboratory Medicine,PLA.Nanjing General Hospital of Nanjing Command； Key Laboratory of Biomaterials and devices of Jiangsu Province,Southeast University；

【摘要】 <正>Objective:To evaluate the effectiveness of Quantum dots(QDs) labeled antibody probe in the applications of indirect immunofluorescence assay(IIFA).Methods:IIFA was used for ANA detection based on QD655-tags,assay results which generated were also compared with that of FITC tags.Results:There existed results discrepancies at a certain degree between QD655- and FITC-tags,including centromere pattern,nuclear homogeneous pattern,nucleolar pattern and cytoplasmic granular pattern,etc,which generated by the target antigen positioned in different parts of the cells.QD655-tags was priority with cytoplasm antigen/antibody reaction,but weakly stained with interphase-cell nuclei,nuclear centromere,dsDNA,nucleosome and histone nuclear-antigens relatively.Yet,ANA results generated by QD655 and FITC fluorescent labellings were roughly approached conformity in split-phase cells.Conclusion:Based on QD-tags,IIFA used for ANA detection is feasible,and has advantages of sensitivity and stability. Synchronously,QD- and FITC-tags together used for ANA will achieve the better effectiveness.更多还原

【Abstract】 Objective:To evaluate the effectiveness of Quantum dots(QDs) labeled antibody probe in the applications of indirect immunofluorescence assay(IIFA).Methods:IIFA was used for ANA detection based on QD655-tags,assay results which generated were also compared with that of FITC tags.Results:There existed results discrepancies at a certain degree between QD655- and FITC-tags,including centromere pattern,nuclear homogeneous pattern,nucleolar pattern and cytoplasmic granular pattern,etc,which generated by the target antigen positioned in different parts of the cells.QD655-tags was priority with cytoplasm antigen/antibody reaction,but weakly stained with interphase-cell nuclei,nuclear centromere,dsDNA,nucleosome and histone nuclear-antigens relatively.Yet,ANA results generated by QD655 and FITC fluorescent labellings were roughly approached conformity in split-phase cells.Conclusion:Based on QD-tags,IIFA used for ANA detection is feasible,and has advantages of sensitivity and stability. Synchronously,QD- and FITC-tags together used for ANA will achieve the better effectiveness.更多还原