A novel population of single cell-derived OCT4~+ stem cells showed increased proliferation and differentiation under hypoxic conditions

【作者】 张少衡； 赵岚； 孙爱军； 邹云增； 葛均波；

【机构】 复旦大学附属中山医院上海市心血管病研究所；

【摘要】 <正>Objective The transcription factor of octamer binding transcription factor 4(Oct4) is considered a main regulator of embryonic stem cell pluripotency and self-renewal capaCIties.Methods By means of FACS,limit-dilution culture,and embryonic stem cell culture conditions,we clonally purified a population stem cell from mouse bone marrow mesenchymal stem cells(MSC).Results These single cell-derived selected MSCs(sMSC) showed homogeneous morphology with normal proliferation and an undifferentiated更多还原