纳米氧化铝颗粒致小鼠学习记忆能力障碍的研究

【作者】 吉俊伟； 张勤丽； 白茹； 高福平； 葛翠翠； 王志武； 张策； 牛侨；

【机构】 山西医科大学教育部省部共建细胞生理学实验室； 国家纳米科学中心纳米生物效应与安全性重点实验室； 中科院理化所纳米材料可控制备与应用研究室；

【摘要】 目的探讨纳米氧化铝颗粒经鼻腔滴注对ICR小鼠学习记忆能力的影响。方法选取经Morris水迷宫实验筛选的学习记忆能力相近的ICR小鼠对其进行不同剂量的纳米氧化铝经鼻腔滴注染毒。染毒后用Morris水迷宫实验、跳台实验和旷场试验检测不同染毒组之间的认知能力,并采用尼氏染色观察海马病理改变。结果纳米氧化铝对小鼠学习记忆能力产生影响,在Morris水迷宫实验中纳米铝组小鼠定位导航潜伏期增加、空间探索实验目标象限停留时间短;跳台实验中纳米铝组小鼠的潜伏期缩短而错误次数增加;旷场试验中纳米铝组小鼠中央格停留时间延长,尼氏染色显示纳米铝组小鼠海马CA3区锥体细胞受损。结论纳米氧化铝颗粒可致小鼠的学习记忆能力损伤,并对海马锥体细胞产生毒性作用。更多还原

【Abstract】 Objective To investigate the inefficiency of cognitive functions in ICR mice exposed through the nasal drip to Nano-alumina particles. Methods After screening with Morris Water Maze (MWM),the mice with similar cognitive abilities were seperated into 6 groups,and exposed to Nano-alumina particles in different concnetrations, Micro-alumina particles,and controls by nasal drip.Cognitive abilities were evaluated with step down test,MWM and open-field test 30d after exposure.Nissl staining was utilized to observe pathological changes in hippocampus.Results Nano-alumina impacts on cognitive ability in mice. Compared with control group,latency of navigation in the mice exposed to Nano-alumina was increased,space exploration experiments target quadrant time was shortened in MWM;the latency of the mice exposed to Nano-alumina was shortened,and the number of errors was increased in step down test;the central cell residence time of the mice exposed to Nano-alumina was increased in Open field test.Nissl staining showed that hippocampal CA3 pyramidal cells were impaired in mice exposed to Nano-alumina.Conclusion Nano-alumina particles can damage cognitive abilities in mice,and have the toxic effects on hippocampal pyramidal cells.更多还原