【作者】 蒋松鹤； 屠文展； 何蓉； 高丽萍； 邹恩苗； 廖维靖；

【Author】 Jiang Song-he;Tu Wen-zhan;He Rong;Gao Liping;Zou En-miao;Liao Wei-jing;Department of Physical Medicine and Rehabilitation,Zhongnan Hospital of Wuhan University;

【机构】 武汉大学中南医院康复医学科； 温州医科大学附属第二医院康复中心；

【摘要】 目的观察单纯针刺和电针对急性脊髓损伤(SCI)大鼠的脊髓组织保护作用是否存在差异。方法采用MASCIS Impactor计算机程控下精确打击制作大鼠SCI模型,选择单纯针刺和电针等方法干预,利用BBB行为评分观察SCI大鼠后肢关节活动、负重、爬行及躯体平衡能力;通过HE染色、尼氏染色等方法观察SCI组织的显微镜下病理形态改变;TUNEL检测损SCI组织细胞凋亡情况;从而分析单纯针刺和电针干预对SCI早期生理病理和功能的影响。结果单纯针刺和电针等在SCI急性期治疗后,BBB评分显示各组在急性期的评分变化很微弱;然与模型组相比,电针组运动能力有所提高。HE染色和尼氏体染色显示假手术组脊髓及神经细胞结构完整,模型组损伤组织周围可见渗出、坏死与水肿,神经元形态显示不规则;单纯针刺和电针等治疗组的神经元的损伤范围介于假手术组和模型组之间。TUNEL显示,模型组大量TUNEL阳性细胞广泛分布于灰质及白质,三组治疗组中TUNEL阳性细胞数量显著下降,细胞凋亡减少,尤其电针组显著。结论单纯针刺和电针均减轻大鼠SCI所致急性损伤,保护脊髓组织,减少SCI后细胞凋亡;其中尤其电针减少的作用较强,且提高早期微弱的运动能力。更多还原

【Abstract】 Objective To screen a stimulation method with the stronger protective effect on spinal cord tissue of acute spinal cord injury(ASCI) rats between the single acupuncture and electroacupuncture(EA).Methods We applied modified Allen’s impact method(MASCIS Impactor,precision strike) to contribute the ASCI rat models.After the administration of the stimulation methods including single acupuncture and EA,we used the BBB score at several time points to evaluate locomotion function changes of ASCI rats;HE staining and Nissl staining to observe the pathological morphology changes of injury spinal cord tissue;TUNEL staining to detect the apoptosis cells of injury spinal cord tissue;to analyze the effects of the three different stimulation methods.Results After treatments of single acupuncture and EA on ASCI rats,BBB score which was administrated at 8.5 hours of postsurgery,showed that locomotion function of rats was promoted in EA group compared with the model group(p<0.01).HE staining and Nissl staining showed that in sham group spinal cord and neurons displayed integrated infrastructures;but exudation,necrosis and edema were seen surrounding damaged tissue with irregular neurons morphology in model group;while the extents of neuronal damage in single acupuncture and EA groups were between that in sham group and that in model group.TUNEL staining showed that the TUNEL-positive cells were widely distributed in both the white and gray matter,with the majority found in the white matter in model group,whereas,the quantities of TUNEL-positive cells in single acupuncture and EA groups significantly decreased,especially in EA group.Conclusion Single acupuncture and EA alleviated the acute injury of rats induced by SCI,protected the spinal cord tissue,and reduced cells apoptosis after SCI,especially EA had the most significant effect,and promoted early locomotion function.更多还原