【作者】 李春华； 赵雅芳； 嵇波； 任晓暄； 郭孟玮； 丁喜艳； 孙琳琳； 张露芬； 朱江；

【Author】 LI Chunhua,ZHAO Yafang,JI Bo,REN Xiaoxuan,GUO Mengwei,DING Xiyan,SUN Linlin, ZHANG Lufen,ZHU Jiang (School of Acupuncture-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029)

【机构】 北京中医药大学针灸推拿学院；

【摘要】 目的:观察电针即刻介入对痛经模型大鼠子宫微循环的影响,进一步探讨针刺缓解胞宫疼痛的作用机理。方法:将动情间期雌性大鼠随机分为盐水组、模型组、三阴交组、悬钟组、非穴组,每组6只。除盐水组外,其余各组大鼠均连续10天给予皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素2u/只,制备痛经大鼠模型,盐水组每日给予同等剂量的生理盐水。于第10日各治疗组给予即刻电针20min。采用XW-B-3冷光源微循环显微检查仪观察各组大鼠子宫微循环不同时间段的变化。结果:模型组微血管、毛细血管(cap)粗细不均,管径收缩,微血管、cap条数减少,血流减慢或停滞;与模型组比,即刻电针三阴交穴20min时,微血管、cap管径均明显扩张,微血管、cap条数均明显增多,差异显著为p<0.05,血流状态无显著性差异为P>0.05;即刻电针悬钟穴、非穴各时间段各指标均无显著性差异为P>0.05,但电针悬钟穴各指标有优于电针非穴的趋势;与非穴组比,电针三阴交20min时,cap管径明显扩张,差异显著为p<0.05。结论:电针即刻介入可缓解子宫血管的痉挛状态,改善子宫微循环,缓解疼痛,且穴位之间存在差异,三阴交>悬钟>非穴。更多还原

【Abstract】 Objective To observe effects of uterus microcirculation of EA on dysmenorrhea rat model, to discuss the mechanism of acupuncture in relieving uterus pain.Methods Diestrus female rats were randomly divided into model group、saline group、EA on Sanyinjiao(SP6) group, Xuanzhong(GB39) group,non-acupoint group,each group had 6 rats.Estradiol benzoate and oxytocin were chosen to be the model of dysmenorrhea.Except saline group,other groups were given subcutaneous estradiol benzoate injection,after the last administration 1h,intra-peritoneal injection of oxytocin 2u /each,Saline group given the same dose of saline per day,On the 10th day,treatment groups were given immediately EA for 20 minutes.Using XW-B-3 Cold Light micro-circulation tester observe the changes of uterus microcirculation at different times.Results The diameter of micro-vessel and cap were shrinked,numbers of micro-vessel and cap were reduced,the blood flow was slow and even stopped.Compared with model group,EA on SP6 for 20minutes,diameters of micro-vessel and cap were significantly expanded,numbers of micro-vessel and cap were significantly increased(p<0.05),the state of blood flow hadn’t significant difference(p>0.05),There wasn’t significant difference in each indicator after EA on GB39、non-acupoint(p>0.05),however,EA on GB39 is better than EA on non-acupoint in each indicator;compared with non-acupoint group,EA on SP6 for 20 minutes,cap diameter was remarkably expanded,p<0.05.Conclusion EA can relieve spasm of uterus blood vessels,improve micro-circulation and relieve pain,there are differences among acupoints,SP6>GB39>non-acupoint.更多还原