【作者】 程珂； 沈雪勇； 丁光宏； 吴凡； 宋花玲；

【Author】 CHENG Ke~(1,2),SHEN Xue-yong~(1,2),DING Guanghong~(2,3),WU Fan~1 (1 Shanghai University of TCM Shanghai 201203,2 Shanghai Research Center of Acupuncture and Meridian Shanghai 200020,3 Fudan University Shanghai 200433 )

【机构】 上海中医药大学； 上海市针灸经络研究中心； 复旦大学；

【摘要】 目的:观察低强度复合和单激光照射急性佐剂性关节炎大鼠足三里穴的镇痛效应及其与肥大细胞脱颗粒效应的关系。方法:采用清洁级SD大鼠66只,随机分为空白组、模型组、假照射组、10.6μm激光照射组、650nm激光照射组和复合激光组,治疗时各照射足三里穴30min。除了空白组,其他5组通过左踝关节腔内注射完全弗式佐剂0.05ml建立急性佐剂性关节炎模型。采用辐射热缩爪反射的方法,以大鼠缩爪反射的潜伏期为观察指标,比较各组的镇痛效果;通过穴位组织切片和甲苯胺蓝染色,离体对照激光照射前后穴位处局部肥大细胞脱颗粒率的变化。结果:激光照射后,650nm组和复合激光组的痛阈显著高于模型组和假照射组(P<0.01),650nm组与复合激光组的肥大细胞脱颗粒率也显著高于模型组和假照射组(P<0.001)。10.6μm组的痛阈和肥大细胞脱颗粒率与模型组、假照射组无显著差异。肥大细胞脱颗粒率与缩爪潜伏期的线性相关系数为0.737(P<0.001)。结论:低强度复合650nm+10.6μm激光和单650nm激光照射急性佐剂性关节炎大鼠足三里穴有显著镇痛效应,穴区中的肥大细胞脱颗粒率与镇痛效应呈正相关,在激光针灸镇痛效应的产生过程中起着重要作用。更多还原

【Abstract】 Objective To observe the analgesia effect of low-level combined-and single-laser irradiation on ST36 in rat and the relationship between mast cell degranulation and laser acupuncture analgesia.Methods A total of 66 Sprague Dawley(SD) rats were randomly assigned into normal control,model control,sham irradiation,10.6μm lase,650nm laser,combined laser(10.6μm+650nm) groups.Arthritis model was established by injection of Complete Freund’s Adjuvant(0.05 mL) into the left ankle joint.The paw withdrawal latency(PWL) was detected with a radiant heat analgesimeter.ST36 was irradiated with sham,single or combined laser for 30 min.After sacrifice under anesthesia(1%embutal),tissues of ST36 area were sampled, sliced(5μm) and stained with Toluidine Blue for skin for observing the mast cell degranulation.Results Compared with model control and sham group,the pain threshold increased significantly in 650nm and combined laser groups(P<0.01),while remained no significant difference in 10.6μm group.Compared with model and sham group,the degranulation ratios of mast cells in 650nm and combined laser group were significantly higher(P<0.001),while remained no significant difference in 10.6μm group.The linear correlation coefficient between degranulation ratios of mast cells and PWL after laser acupuncture is 0.737(P<0.001). Conclusion Both 650nm and combined laser stimulation of ST36 can effectively raise pain threshold,and enhance degranulation ratio of mast cells at the stimulated acupoint.The result also suggested a linear correlation between degranulation ratio of mast cell and analgesia effect.更多还原