【作者】 孙铮； 战丽彬； 姜如娇； 解伟；

【Author】 Sun Zheng~1,Zhan Libin~(1,2),Jiang Rujiao~1,Xie Wei~1 1.College of Integrative Medicine and Institute of Integrative Medicine,Dalian Medical University,Dalian 116044,China; 2.The Second Affiliated Hospital of Dalian Medical University,Dalian 116023,China

【机构】 大连医科大学中西医结合学院(研究院)； 大连医科大学附属二院；

【摘要】 目的:观察滋补脾阴方药对糖尿病认知功能障碍大鼠大脑皮质保护作用及其线粒体相关机制。方法:将SD大鼠随机分为空白对照组(Con),糖尿病组(DM),滋补脾阴方药组(ZBPYR)三组。透射电镜、JC-1染色标记法对大鼠皮质组织线粒体超微结构、数量和线粒体膜电位(△ψm)变化进行了线粒体功能评价;Western blotting观察细胞色素C(CytoC)、电压依赖性离子通道(VDAC)的表达,分析ZBPYR的线粒体保护机制。结果:与Con组比较,DM组线粒体数量增多、肿胀、变形、嵴走向紊乱,模糊或溶解,甚至结构完全破坏,ZBPYR组可明显减轻上述病理改变;DM组较Con组△ψm水平降低(P<0.01),与DM组比较,ZBPYR组△ψm水平升高(P<0.05);DM组胞浆中Cyto C表达明显高于Con组和ZBPYR治疗组(P<0.01),线粒体Cyto C显著低于Con组和ZBPYR组(P<0.01)。结论:ZBPYR发挥对DM大鼠大脑皮质保护作用可能与改善线粒体形态、数量的变化、抑制线粒体膜电位降低,阻止Cyto C向胞浆中释放有关。更多还原

【Abstract】 Objective:To investigate the protection of Zibu Piyin Recipe(ZBPYR) on the mitochondrial injury in the prefrontal cortex of deficiency diabetic encephalopathy rats.Methods: SD rats were randomly divided into three groups(n=10 each):the control group(Con group),the diabetes group(DM group) and the ZiBu PiYin Recipe treatment group(ZBPYR group).TEM and JC-1 labeling were used to test the ultrastructure,quantity and membrane potential(△Ψm) of mitochondria in prefrontal cortex of SD rats;Western blot analysis was used to observe die expression of cytochrome C(Cyto C) and voltage-dependent ion channels(VDAC) before and after ZBPYR treatment.Results:Compared with the Con group,the number of mitochondria in DM group was increased and the structure showed disorder and blurred,even completely destroyed.While in ZBPYR group these pathological changes were reduced obviously.The△Ψm levels of DM group was decreased than that in the Con group(P<0.01),while compared with DM group,the△Ψm levels of ZBPYR group was increased(P<0.05).The expression of Cyto C in cytoplasm of DM group was significantly higher than ZBPYR group,however,the expression of Cyto C in mitochondrial was significantly lower than Con and ZBPYR group(P<0.01). Conclusion:ZBPYR plays a vital role in the protection of DM in rat cerebral cortex and the mechanism of it may be associated with improvement of mitochondrial morphology,changes of volume,inhibition of mitochondrial membrane potential,and prevention the release of Cyto C from cytoplasm.更多还原