【作者】 沈頔； 邢新； 王晓云； 徐达圆； 杨超；

【Author】 SHEN Di,XING Xin,WANG Xiaoyun,XU Dayuan, YANG Chao, Department of Plastic Surgery,Changhai Hospital Affiliated the Second Military Medical University,Shanghai

【机构】 第二军医大学附属长海医院整形外科；

【摘要】 目的:研究环孢素A对瘢痕疙瘩成纤维细胞增殖及凋亡的影响。方法:用环孢素A作用于体外培养的瘢痕疙瘩成纤维细胞。Annexin V-FITC/PI流式细胞术检测成纤维细胞凋亡率,DAPI染色观察成纤维细胞的凋亡形态,CCK-8比色法检测环孢素A对成纤维细胞增殖的影响并计算半数抑制浓度。结果:随着环孢素A浓度增高,成纤维细胞凋亡率不断增高,差异具有统计学意义。通过数据分析获得的半数抑制浓度为(1.908±0.280)g/L。结论:环孢素A对瘢痕疙瘩成纤维细胞的生长增殖具有抑制作用,并有望在瘢痕疙瘩的防治中发挥重要作用。更多还原

【Abstract】 Objective To study the biological effect of cyclosporin A on the proliferation and Apoptosis of fibroblasts in Keloid.Methods Cyclosporine A was used on cultured keloid fibroblasts,then we used Annexin V-FITC/ PI flow cytometry for the rate of fibroblast apoptosis,DAPI staining for the apoptotic fibroblasts form.CCK-8 colorimetric for the half inhibitory concentration.Results With the increasec concentration of cyclosporin A,the apoptosis rate constantly increased with a statistically significant difference.Data analysis was obtained by the half inhibitory cor centration(1.908±0.280) g / L. Conclusion Cyclosporin A inhibit the proliferation of Fibroblasts in Keloid,and is expected to play an important role in the prevention of keloid更多还原