【作者】 张宇； 严际慎； 张勤； 张琪； 裘华森；

【Author】 Yu Zhang,Qin Zhang,Qi Zhang,Hua-Sen Qiu.Department of General Surgery,Zhejiang Provincial Chinese Traditional Medicine Hospital.Hangzhou 310006,Zhejiang Province,China Ji-Shen Yan,Department of General Surgery,Renmin Hospital of Wuhan University,Wuhan 430071,Hubei Province,China

【机构】 Department of General Surgery,Zhejiang Provincial Chinese Traditional Medicine Hospital； Department of General Surgery,Renmin Hospital of Wuhan University；

【摘要】 目的:探讨血清可溶性细胞间粘附分子-1(sICAM-1)水平变化与重症急性胰腺炎(SAP)肺损伤的关系方法:SD大鼠采用逆行胰胆管注射30 g/L牛磺胆酸钠方法制作SAP模型取0,3,6,12,24 h不同时点血清进行sICAM-1检测,同时检测肺组织髓过氧化物酶活性.结果:制模3 h后,血清sICAM-1水平开始持续上升(34.90-38.99■mol/L),肺组织髓过氧化物酶(MPO)活性在6 h后明显增强(P<0.05),血清sICAM-1水平与肺泡肺组织MPO活性呈正相关(r=0.897).结论:重症急性胰腺炎时血清sICAM-1水平的变化可能与肺部损伤程度密切相关.更多还原

【Abstract】 AIM:To investigate the relationship between the concentration change of soluble intercellular adhesion molecule-1(sICAM-1) in severe acute pancreatitis(SAP) and associated lung injury in rat. METHODS:Rat SAP model was established by retrograde injection of 3%sodium taurocholate into bile-pancreatic duct.At different time points of 0,3,6,12,24 hours,we sacrificed rats and collect their sera and left lung.Sera sIC AM-1 and the myeloperocidase(MPO)activity of lung tissue were measured. RESULTS:The increases of sera sICAM-1 concentrations were evident at 3 hours after SAP induced,and the myeloperocidase(MPO) activity of lung tissue were significantly increased at 6 hour.Sera sICAM-1 concentration positively correlation with the myeloperocidase(MPO) activity(r=0.897). CONCLUTION:The concentration change of serum sICAM-1 is associated with the pathological injury magnitude of lung in rat severe acute pancreatitis.更多还原