【作者】 张振辉； 林珮仪； 陈晓辉； 江慧琳； 李艳玲； 王华军；

【Author】 ZHANG Zhenhui,LIN Peiyi,CHEN Xiaohui,JIANG Huilin,LI Yanling,WANG Huajun,Intensive Care Unit, The Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China

【机构】 广州医学院第二附属医院危重病学科；

【摘要】 目的:探讨巨噬细胞移动抑制因子(MIF)在脓毒症小鼠心脏和肾组织中的表达规律。方法:采用盲肠结扎穿孔术(CLP)建立脓毒症BALB/c小鼠模型。30只小鼠随机分为5组,分别为假手术组,CLP后12、24、36、48h组,其中假手术组小鼠在假手术后24小时取标本检测作为对照组,其余四组小鼠分别在CLP术后的12h、24h、36h和48h取标本待检。采用逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western Blotting)检测脓毒症小鼠心脏和肾组织中MIF的mRNA和蛋白表达。采用单因素方差分析(one-way ANOVA)方法进行计量资料的统计分析。结果:与对照组比较,MIF在脓毒症小鼠心脏组织中的表达增加,在CLP术后12h开始升高(P<0.05),36h达峰值(P<0.01),48h仍维持较高水平(P<0.05);肾组织中MIF mRNA的表达在CLP术后12h开始增加(P<0.05),24h达峰值(P<0.01),48h后才开始减少,MIF蛋白则只在CLP术后24h和36h增加明显(P<0.05)。结论:脓毒症发生后的12h～48h,MIF在脓毒症小鼠心脏和肾组织中的表达有不同程度增加,表达时相基本一致,提示MIF可作为晚期细胞因子参与小鼠脓毒症的发病。更多还原

【Abstract】 Objective:To investigate the expression profile of MIF in heart and kidney tissues of sepsis mice. Methods:The sepsis model was induced by Cecal ligation and puncture(CLP) in mice.Thirty male BALB/c mice were randomly divided into five groups:sham operation group,the twelfth hour,twenty-forth hour,thirty-sixth and forty-eighth hour group after CLP.MIF mRNA were semiquantitated by the reverse transcription polymerase chain reaction(RT-PCR),Western Blotting was performed for MIF protein.Measurement data was analyzed with one-way ANOVA.Results:MIF mRNA and protein expression in heart tissues significantly increased at the twelfth hour,peaking at the thirty-sixth hour,and a high level was maintained till the forty-eighth hour after CLP. But in kidney tissues of models,the content of MIF reached peak at the twenty-forth hour and started to decrease at the forty-eighth hour after CLP.Conclusions:The content of MIF in heart and kidney tissues of sepsis models was higher than sham group,especially from the twelfth hour to forty-eighth hour after CLP.It indicated that MIF as a kind of late cytokine might participate in impairment of organs in mice with sepsis.更多还原