【作者】 于杰； 赵丕文； 牛建昭； 王继峰； 郝庆秀；

【Author】 YU Jie,ZHAO Piwen,NIU Jianzhao~*,WANG Jifeng,HAO Qingxiu (Beijing University of Chinese Medicine,Beijing 100029,China)

【机构】 北京中医药大学；

【摘要】 目的:检测胶艾汤及参芪胶艾汤对小鼠子宫ER亚型表达的影响,研究中药方剂的雌激素样作用。方法:正常昆明种性未成熟雌性小鼠60只,按体重均衡和随机的原则分为6组:正常组、阳性药己烯雌酚组、胶艾汤和参芪胶艾汤低,高剂量组;灌胃持续4d,取子宫进行石蜡包埋和切片。通过免疫组化染色法检测灌胃药物后小鼠子宫组织ERα和ERβ表达的改变情况。结果:(1)低剂量胶艾汤可明显诱导子宫ERα表达增强(P<0.01),强度与己烯雌酚相似;低剂量和高剂量参芪胶艾汤均有诱导ERα表达增强的作用(P<0.01),强度弱于己烯雌酚组;(2)在诱导ERβ表达方面,己烯雌酚可诱导子宫ERβ表达明显增强,低剂量胶艾汤组诱导ERβ表达的作用强于己烯雌酚组(P<0.01),高剂量参芪胶艾汤也具有增强ERβ表达的作用,但程度弱于己烯雌酚(P<0.05)。结论:胶艾汤及其加味方参芪胶艾汤具有一定的雌激素受体亚型特异性的雌激素样作用。更多还原

【Abstract】 Objective:To study the phytoestrogenic effects of Jiaoaitang and Shenqi jiaoaitang through the tests of expression of estrogen receptor subtypes in mice.Method:Sixty kunming mice weighing 9-12 g were randomly divided into 6 groups:solvent control group, diethylstilbestrol control group and 4 Chinese medicine treated groups(administrated low or high doses of Jiaoaitang and Shenqi jiaoaitang respectively).After administration for 4 days,the mice were sacrificed;uterus was removed and embedded in paraffin.ERαand ERβexpression was tested by immunohistochemical technique.Result:Administration of Jiaoaitang at low dose significantly increased ERαexpression in uterus(P<0.01).Both Shenqi jiaoaitang at low or high dose could also induce elevation of ERαexpression obviously(P<0.01).Administration of Jiaoaitang at low dose or Shenqi jiaoaitang at high dose can cause elevation of ERβlevel(P<0.05 or 0.01).Conclusion: Jiaoaitang and Shenqi jiaoaitang have phytoestrogenic effects and their effects are ER subtype specific.更多还原