【作者】 关伟； 朱建华； 戴清保；

【Author】 GUAN wei~1,DAI qing-bao~2,WU xiao-qiao,ZHU jian-hua(1.Department of Forensic Toxicology,Wannan Medical University,Wuhu 241002,China;2.People’s County of Yingshang,Anhui Yingshang 236200)

【机构】 皖南医学院法医毒理毒化教研室； 颍上县人民法院；

【摘要】 目的本实验观察雷公藤甲素对大鼠脑皮质Fas蛋白表达的影响并探讨其时间节律性。方法雄性SD大鼠40只随机分为4组,包括空白对照组;雷公藤甲素Ⅰ组20μg·kg^-1.bid（8am,8pm）;雷公藤甲素Ⅱ组40μg·kg^-1.qd.8am;雷公藤甲素Ⅲ组40μg·kg^-1.qd.8pm。连续灌胃7周后断头处死,用免疫细胞化学染色法观察大鼠脑皮质Fas蛋白的表达变化。结果雷公藤甲素Ⅰ组大鼠脑皮质FAS蛋白的免疫反应的表达最高,雷公藤甲素Ⅲ组FAS蛋白的免疫反应仅次于雷公藤甲素Ⅰ组,而雷公藤甲素Ⅱ组仅可见少量表达;空白对照组未见大鼠脑皮质FAS蛋白的免疫反应。结论雷公藤甲素可通过死亡受体介导细胞凋亡的途径诱导细胞凋亡,从而发挥其抗肿瘤、免疫抑制作用。且雷公藤甲素的作用具有时间节律性。更多还原

【Abstract】 Objective To observe the effect of triptolide on the Fas protein expression in rat brain cortex and to explore the time rhythm.Methods 40 Male SD rats were randomly divided into 4 groups, including the normal control group;triptolide groupⅠ(triptolide 20μg·kg-1.bid,Time:8am and 8pm); TriptolideⅡgroup(triptolide 40μg·kg-1.qd,Time:8am);triptolideⅢgroup(triptolide 40μg·kg-1.qd,Time:8pm).Decapitation of SD rates after gavage for 7 weeks,observed the Fas protein expression in rat brain cortex by immune cells staining.Results In triptolide GroupⅠ,markedly highest expression of the immune response of Fas protein in rat brain cortex than other groups;triptolide groupⅢsecond;a small amount of expression in triptolideⅡgroup;and no expression of FAS protein immune response in control group.Conclusion On basis of the death receptor-mediated apoptosis triptolide can induce apoptosis,play its role in anti-cancer and immune inhibition.And the effect of triptolide can be marked by features of time rhythm.更多还原