【作者】 王爱民； 周国燕；

【Author】 Wang Aimin,Zhou Guoyan (Institute of Cryo-medicine and Food Refrigeration,Shanghai University of Science and Technology, Shanghai 200093,China)

【机构】 上海理工大学低温医学与食品冷冻研究所；

【摘要】 低温保存寄生虫可消除传统保存方法的弊端,并可保持寄生虫原有的生物学特性。本文综述了寄生原虫和蠕虫低温保存的研究现状,着重从低温抗冻剂、降温过程和复温过程三个方面,探讨了不同寄生虫低温保存的最佳工艺,分析得出低温抗冻剂、冷冻速度和融冻速度是影响保存损伤程度的主要因素,为寄生虫低温保存研究提供理论帮助,并指出今后研究的方向。更多还原

【Abstract】 The cryopreservation of parasites not only can eliminate limitations,but also can maintain the original biological characteristics of parasites.In this paper,the research progress of cryopreservation of protozoa and worms parasites is summarized,of which the cryoprotectant,freezing processes and thawing processes are mainly discussed.And the conclusion is got that the most important factors of cryopreservation of parasites are cryoprotectant,freezing and thawing rates.Some key subjects of further research are pointed out.更多还原