【作者】 马涛； 袁力勇； 王丹； 戴体俊； 郭忠民； 张励才；

【Author】 MA tao~1,YUAN Li-yong,WANG dan~1,DAI Ti-jun~1,GUO Zhong-min~1,Zhang Li-cai~1 (1.Anesthesia college of Xuzhou Medical College,Xuzhou Jiangsu 221004,China;2.Anesthesiology department of Ningbo Women and Children Hospital,Ningbo Zhejiang 315012,China)

【机构】 徐州医学院麻醉学院； 宁波市妇女儿童医院麻醉科；

【摘要】 目的探讨异氟烷（isoflurane,Iso）镇痛作用是否与激动小鼠脊髓5-HT_1A受体有关。方法使用甩尾法、热板法、扭体法。3种方法共筛选出240只小鼠,每种方法80只,均分为以下8小组:生理盐水组（NS组）,异氟烷组（Iso组）,单用不同剂量5-HT_1A体拮抗剂p-MPPF组(MPPF₆组、MPPF₃组、MPPF_1.5组),合用药组(Iso+MPPF₆组、Iso+MPPF₃组、Iso+MPPF_1.5。甩尾法以甩尾潜伏期,热板法以舔后足潜伏期,扭体法以15分钟内扭体次数为指标,观察鞘内注射（intrathecal injection,i.t）p-MPPF对Iso镇痛作用的影响。结果单独i.t 6μg、3μg、1.5μgp-MPPF对上述观察指标无明显影响（p>0.05）。与Iso组相比,Iso+MPPF₆组的甩尾潜伏期和舔后足潜伏期均缩短（p<0.01）,15分钟内的扭体次数增多（p<0.05）;Iso+MPPF₃组甩尾潜伏期和舔后足潜伏期均缩短（p<0.01或p<0.05）,扭体次数无明显变化（p>0.05）;Iso+MPPF_1.5组的三种观察指标均无统计学差异（p>0.05）。结论吸入麻醉药Iso镇痛作用与激动脊髓5-HT_1A受体密切有关。更多还原

【Abstract】 Objective The present study was designed to investigate whether isoflurane（Iso） produces the analgesic effects by exciting the 5-HT_1A receptor in the spinal cord of mice.Methods Tail-flick test, hot-plate test and acetic-acid-writhing test were used.Each test required 80 mice that meet the requirements so there was a total of 240 mice.80 mice were divided into the following eight groups:NS group,isoflurane group（Iso group）,p-MPPF(5-HT_1A receptor antagonist) alone in three different dose groups(MPPF₆ group,MPPF₃ group,MPPF_1.5 group),combined treatment groups(Iso+MPPF₆ group, Iso+MPPF₃ group,Iso+MPPF_1.5 group).The tail-flick latency（TFL） in tail-flick test,the pain threshold in hot-plate test（HPPT）,writhing times in 15 minutes as indicators to observe the impacts of p-MPPF intrathecally（i.t） on the analgesic effects of Iso.Results I.t 6μg,3μg,1.5μg p-MPPF alone had no effects on the observed indicators（p>0.05）;Compared with Iso group,the TFL and HPPT of Iso+MPPF₆ group were shortened（p<0.01）,the writhing times in 15 minutes were increased（p<0.05）; The TFL and HPPT of Iso+MPPF₃ group had been shorten（p<0.01 or p<0.05） but writhing times had no significant changes（p>0.05）;the three kinds of indicators of Iso+MPPF_1.5 had no statistical difference （p>0.05）.Conclusion These findings suggeste that the analgesic effects of Iso are closely related to the 5-HT_1A receptor in the spinal cord.更多还原