【作者】 陈小兵； 罗素霞； 张军辉； 刘晓莉； 黄幼田； 索振河；

【Author】 Chen Xiaobing~(1,2) Luo Suxia~(1,2) Zhang Junhui~1 Liu Xiaoli~2 Huang Youtian~3 Suo Zhenhe~1 1 the First Affiliated Hospital Zhengzhou University,Zhengzhou,Henan Province 450052 2 Henan Province Tumor Hospital,Henan Province,Zhengzhou,Henan Province 450008 3 Department of Pathophysiology,Basic Medical College,Zhengzhou University,Zhengzhou, Henan Province 450052

【机构】 郑州大学第一附属医院； 河南省肿瘤医院； 郑州大学基础医学院病理生理教研室；

【摘要】 目的:探讨靛玉红甲肟（Indirubin-3’-monoxime）对体外培养的人食管癌细胞系EC-1和Kyse70增殖和细胞周期的影响。方法:不同浓度靛玉红甲肟处理培养的食管癌EC-1和Kyse70细胞后,采用MTT法检测细胞的增殖活性,流式细胞仪和RT-PCR检测细胞周期分布及bcl-2和bax mRNA的变化。结果:靛玉红甲肟对人食管癌细胞EC-1和Kyse70的生长具有明显的抑制作用,其作用表现为剂量依赖性和时间依赖性（P<0.05）。流式细胞仪检测发现:以5μmol/L,的靛玉红甲肟对EC-1细胞处理24、48、72 h后,G₀/G₁期细胞比例逐渐下降（P<0.05）,S期细胞比例未见明显改变,G₂/M期细胞比例逐渐上升（P<0.05）,呈时间依赖性。RT-PCR检测发现人食管癌细胞bcl-2和bax mRNA比例呈时间依赖性下调。结论:靛玉红甲肟对人食管癌细胞系EC-1和Kyse70具有明显的增殖抑制作用,其作用机制与阻滞细胞周期G₂/M期和下调bcl-2/bax表达比例有关。更多还原

【Abstract】 Objective:To observe the effect of Indirubin-3’-monoxime on proliferation and cell cycle of human esophageal cancer cell line EC-1 and Kyse70.Methods:EC-1 and Kyse70 cells were treated with Indirubin-3’-monoxime.The proliferative status of cells was measured by methabenzthiazuron（MTT） assay.Cell cycle and expression of bcl-2,bax mRNA was analyzed, detected by flow cytometry（FCM） and RT-PCR.Results:Indirubin-3’-monoxime inhibited growth of EC-1 and Kyse70 cells in a dose-dependent and time-dependent manner（P<0.05）.Cell G₀/G₁ phase ratio decreased gradually（P<0.05）,S-phase ratio has not changed significantly,G₂/M phase ratio increased gradually after exposed to Indirubin-3’-monoxime 24、48、72h in a dose dependent manner（5μmol/L）（P<0.05）,RT-PCR detection of esophageal cancer cells were found in bcl-2 and bax mRNA ratio down in a dose dependent manner（5μmol/L）（P<0.05）.Conclusions: Indirubin-3’-monoxime inhibits the proliferation in human esophageal cancer line EC-1 and Kyse70.The effect mechanism is related to arrest the G₂/M phase in cell cycle and bcl-2 and bax mRNA ratio down.更多还原