Functional characterization of Schizosaccharomyces pombe DJ-1 and Hsp31 proteins,homologs of the Parkinson’s disease protein DJ-1

【作者】 苏杨； 赵巧巧； 王志康； 陈彩萍； 吴桐思雨； 黄鹰；

【Author】 Jiangsu Key Laboratory for Microbes and Genomics,Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources,School of Life Sciences,Nanjing Normal University;

【机构】 Jiangsu Key Laboratory for Microbes and Genomics,Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources,School of Life Sciences,Nanjing Normal University；

【摘要】 <正>Reactive carbonyl species(RCS),such as methylglyoxal(MG)and glyoxal(GO),are synthesized as toxic metabolites in living systems.Mechanisms of RCS detoxification include the glutathione(GSH)-dependent system consisting of glyoxalase I(GLO 1)and glyoxalaseⅡ(GLO2),and GSH-independent system involving glyoxalaseⅢ(GLO3).Hsp31 and DJ-1 proteins are weakly homologous to each other and belong to two different subfamilies of the DJ-1/Hsp31/PfpI superfamily.Recently,the Escherichia coli Hsp31 protein and the D J-1 proteins from Arabidopsis thaliana and metazoans have been demonstrated to have GLO3 activity.We performed a更多还原