OsPTR9 affects nitrogen utilization efficiency,growth,and grain yield in rice

【作者】 FANG Zhong-ming； 张明永；

【Author】 FANG Zhong-ming,ZHANG Ming-yong~* Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden, Chinese Academy of Sciences,Guangzhou 510650

【机构】 Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences；

【摘要】 <正>The plant NPF family(previously known as PTR/NRTI:peptide transporter/nitrate transporter 1)comprises di/tripeptide and low affinity nitrate transporters;some members also recognize other substrates such as carboxylates,phytohormones(auxin and abscisic acid),or defense compounds(glucosinolates).Little is known about the members of this gene family in rice (Oryza sativa L.).We report the influence of altered OsPTR9 expression on nitrogen utilization efficiency,growth,and grain yield.OsPTR9 expression is regulated by exogenous nitrogen and by the day-night cycle.Transient expression of OsPTR9-eGFP in onion epidermal cells and rice showed OsPTR9 localized to the plasma membrane.RNAi and osptr9 mutant showed that OsPTR9 affects lateral root development,the vasculature development in stems,plant growth and ammonium uptake.Elevated expression of OsPTR9 in transgenic rice plants resulted in enhanced ammonium uptake,promotion of lateral root formation,and increased grain yield.These results suggest that OsPTR9 might hold potential for improving nitrogen utilization efficiency and grain yield in rice breeding更多还原

【Abstract】 The plant NPF family(previously known as PTR/NRTI:peptide transporter/nitrate transporter 1)comprises di/tripeptide and low affinity nitrate transporters;some members also recognize other substrates such as carboxylates,phytohormones(auxin and abscisic acid),or defense compounds(glucosinolates).Little is known about the members of this gene family in rice (Oryza sativa L.).We report the influence of altered OsPTR9 expression on nitrogen utilization efficiency,growth,and grain yield.OsPTR9 expression is regulated by exogenous nitrogen and by the day-night cycle.Transient expression of OsPTR9-eGFP in onion epidermal cells and rice showed OsPTR9 localized to the plasma membrane.RNAi and osptr9 mutant showed that OsPTR9 affects lateral root development,the vasculature development in stems,plant growth and ammonium uptake.Elevated expression of OsPTR9 in transgenic rice plants resulted in enhanced ammonium uptake,promotion of lateral root formation,and increased grain yield.These results suggest that OsPTR9 might hold potential for improving nitrogen utilization efficiency and grain yield in rice breeding更多还原