【作者】 颜景斌； 潘树标； 龚秀丽； 郭歆冰； 任兆瑞； 陈美珏； 曾溢滔；

【Author】 Jingbin Yan,Shubiao Pan,Xiuli Gong,Xinbing Guo,Zhaorui Ren,Chen Meijue,Yitao Zeng. Shanghai Institute of Medical Genetics,Children’s Hospital of Shanghai,Shanghai Jiao Tong University

【机构】 上海交通大学医学遗传研究所；

【摘要】 <正>Transferrin(TF),an iron-binding glucoprotein,plays complex physiological roles related to cell function,differentiation and proliferation.Previous studies indicated that the expression of TF gene was regulated by methylation at cellular level.To explore the relationship between methylation and gene expression at individual level,we generated a number of human TF transgenic mice,in which hTF gene expression was driven by rabbit TF promoter and enhancer.The structure analysis showed that abundant CpG sites were within the rabbit TF promoter.The transgenic mice with single copy of the transgene were selected to determine the hTF transcripts in different tissues.Meanwhile,the更多还原