【作者】 云宇光； 娜琴； 崔明玉； 陈阿梅；

【Author】 Yun yu-guang Na qin Cui ming-yu,Chen a-mei

【机构】 内蒙古医学院组织学与胚胎学教研室； 内蒙古人民医院；

【摘要】 目的分析人类白细胞抗原(human leukocyte antigen,HLA)HLA-A、B、基因多态性与内蒙地区白血病的相关性。方法采用Luminex流式技术-序列特异性寡核苷酸探针反向杂交(flow cytometry-sequence specificoligonucleotide probe,FLOW-SSOP)技术对内蒙地区87例白血病患者HLA-A、B等位基因多态性进行分析,以北方地区健康群体资料作为正常对照。等位基因频率采用直接计数法,组间比较用X2检验。结果 (1)在HLAA等位基因中,白血病组中HLA-A*11XX,*31XX,*6601基因频率(11.917%,3.51%,0.288%)明显高于对照组(8.133%,1.955%,0.03%),二者有显著性差异(P<0.05);HLA-A*33XX基因频率(2.032%)明显低于对照组(5.825%),二者差异显著(P<0.05)。(2)在HLA-B等位基因中,白血病组中HLA-B*15XX(72),*27XX,*38XX,*49XX,*59XX基因频率(0.288%,2.326%,3.808%,0.288%,0.576%)高于对照组(0.01%,0.968%,0.702%,0.051%,0.03%),差异有统计学意义(P<0.05);HLA-B*4801,*58XX基因频率(0.288%,1.447%)低于对照组(1.728%,3.435%),差异有统计学意义(P<0.05)。结论内蒙地区白血病的发生与HLA-A*11XX,*31XX,*6601,B*15XX(72),*27XX,*38XX,*49XX,*59XX等位基因呈正相关;HLA-A*33XX,B*4801,*58XX,等位基因与白血病发生可能有遗传拮抗作用。更多还原

【Abstract】 [Objective]To explore the correlation between the HLA -A,-B alleles and susceptibility to leukemia in Inner Mongolin.Methods HLA-A、B alleles polymorphism in patient of 87 were examined with flow cytometry-sequence specific oligonucleotide probes(FlOW-SSOP) methods.2516 normal subjects in Northern China as control.The allelic frequencies were calculated,and statistical differences between study groups were identified by use of the X2 test.Results(1)The frequencies of allele HLA-A*11XX,*31XX,*6601were increased(P<0.05);while the frequencies of allele HLA-A*33XXwere decreased in patients with Leukemia(P<0.05).(2)The frequencies of allele HLA-B*15XX(72),*27XX,*38XX,*49XX,*59XX were increase(P<0.05);while the frequencies of allele HLA-B *4801,*58XX were decreased in patients with Leukemia(P<0.05)Conclusion HLA-A*11XX,A*31XX,A*6601, B*15XX(72),B*27XX,B*38XX,B*49XX,B*59XX,alleles may be positive correlated with Leukemia,while HLA-A *33XX,B*4801,B*58XXseem to contribute to its genetic resistance.These results suggested that positive association may exist between certain HLA genes and leukemias.更多还原