【作者】 申莉莉； 王凤龙； 钱玉梅； 杨金广； 陈德鑫；

【Author】 Shen lili, Wang fenglong, Qian yumei, Yang jinguang, Chen dexin (Tobacco Research Institute, CAAS, Qingdao, 266101)

【机构】 中国农科院烟草研究所；

【摘要】 以解淀粉芽孢杆菌By33分泌蛋白为试验对象,在Nc89上接种TMV6h后,接种叶片悬浮于活性蛋白不同稀释浓度及无菌水中48h后,取出做摩擦接种三生烟试验和ELISA检测病毒含量试验。结果表明:活性蛋白稀释10倍、25倍、50倍对TMV有抑制体内复制的作用,摩擦枯斑试验抑制率分别为94.4%、93.0%、61.9%,随稀释倍数增加,抑制率逐渐降低,稀释100倍、250倍对TMV体内复制失去抑制作用,测定结果与ELISA检测病毒含量结果一致。更多还原

【Abstract】 The article studied the inhibitory effect of active protein of Bacillus amyloliquefaciens against TMV replication in tobacco leaves. Six hours after inoculation TMV on host tobacco Nc89, removing the inoculated leaves and floating in the active protein and sterile water, 48 hours later, one half sample leaves used for inoculation on Sam sum NN, the other half used for ELISA. The results showed that, active protein of 10,25 and 50 multiple dilution had inhibitory effect of 94.4%、93.0%、61.9% respectively. The effect got decrease as the multiple dilution increase, the same to the result of ELISA.更多还原