【作者】 张莹； 金伟； 吕绍武； 牟颖；

【Author】 Zhang Ying1 Jin Wei1 Lv Shaowu2 Mu Ying1,2(1.Research Center for Analytical Instrumentation,State Key Lab of Industrial Control Technology,Institute of Cyber-Systems and Control,Zhejiang University,Hangzhou 310058,PR China 2.Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education,Jilin University,Changchun 130023,PR China)

【机构】 浙江大学工业控制技术国家重点实验室,智能系统与控制研究所； 浙江大学分析仪器研究中心； 吉林大学分子酶学工程教育部重点实验室；

【摘要】 使用表面等离子体子共振(surface plasmon rensonance,SPR)生物传感器研究谷胱甘肽过氧化物(Glutathione peroxidase,GPX)模拟酶与其底物谷胱甘肽(Glutathione,GSH)的相互作用,其中GSH既是GPX模拟酶的底物同时又可在传感器金膜表面进行自组装形成敏感层。本文中使用的生物传感器属于波长检测型,测定了三种GPX模拟酶与底物相互作用的动力学参数,其中6-CySeCD与GSH的相互作用要强于2-TeCD和2-SeCD,其结合常数为3.25×105L·mol-1。因此对于相关有GPX效应的模拟酶的筛选是十分有价值的。此方法简单、快速、灵敏,而且可降低成本,无需标记。更多还原

【Abstract】 A method for the study of interaction of glutathione peroxidase(GPX) mimics with substrate GSH on surface plasmon resonance biosensor that utilizes GSH as self-assembled monolayer is developed.This biosensor is based on wavelength-detected.The information of kinetics on interaction between GPX mimics and GSH is analylized according to wavelength shift by biosensor.6-CySeCD is the stronger than 2-TeCD and 2-SeCD for being combined with GSH,and its association contant(Ka) based on Langmuir adsorption isotherm is 3.25×105 L· mol-1.GPX is known of its ability of antioxidant and scavenging reactivity,so the study of GPX mimics is very meaningful.The SPR method used to detect and get information of interaction between GPX mimics and GSH is simple,fast,sensible and low-cost,free label.更多还原