【作者】 王琼； 李翊华； 陈善广； 朱轶范； 刘新民；

【Author】 WANG Qiong~(1,5),LI Yi-hua~2,CHEN Shan-guang~3,ZHU Yi-fan~4,LIU Xin-min~1 (1.Chinese Academy of Medical Sciences & Peking Union Medical College,Institute of Medicinal Plant Development,Beijing 100193;2.DaHen Group,Chinese Academy of Sciences,Beijing 100086;3.the Scientific Research and Training Center for Chinese Astronauts,Beijing 100094;4.Beijing 3D CT&DR Technology Corp;Beijing 100007;5.Luzhou Medical College,Luzhou 646000,China)

【机构】 中国医学科学院&北京协和医学院药用植物研究所； 泸州医学院； 中国科学院大恒集团； 中国航天员科研训练中心； 北京三维拓盟数字影像技术有限公司；

【摘要】 目的将计算机、信息和图像分析技术集成用于动物自主活动实验中,在线分析获取大小鼠自主活动的信息资源;方法采用模式识别、数据挖掘等技术对信息资源进行科学组合和深层次开发,提取包括运动距离、运动时间、运动速度、静息时间及各种比值以及运动轨迹等快速灵敏、规范客观的评价指标;并利用正常小鼠、安定和咖啡因造模的小鼠对指标系统进行可靠性验证;结果采用以上方法建立的自主活动实时测试分析处理系统,稳定可靠,所获数据与原人工检测方法所获结果具有高度相关性,相关系数在0.9以上;安定和咖啡因实验验证显示,系统可靠性高;结论全套系统集各种信号监测、采集、自动判别、贮存、结果分析、统计处理和输出一体化,为镇静、抗抑郁等神经精神中药药理实验研究提供了一套自动化程度高、提取信息量大、可供推广应用的空场实验计算机在线图像检测分析处理系统。更多还原

【Abstract】 Combining the advantage of computer sciences,information knowledge,and image analysis technique into open field test, the data of mice locomotor activities were collected and analysis with technique of photography,multimedia,pattern-recognition, data mining etc.Various parameters such as total Distance of Movement(TDM),Movement Speed(MS),Total Duration of Movement(TDM),Movement Distance in Peripheral Area/Central Area(MDPA/MDCA),Movement Time in Peripheral Area/Central Area(MTPA/MTCA),Total Resting Duration(TRD),Resting Duration in Peripheral Area/Central Area (RDPA/RDCA) etc.were recorded after data was collected,refined and analyzed with self-designed software.Experimental results showed those indexes are more sensitive and more objective for judging animal locomotor in open field,after mice were administrated intra-gastric ally with diazepam,caffeine.The correlation coefficient of those indexes collected by computer and manpower were higher than 0.9 after more than 500 mice were experimented.It provides an automatic,intelligent system for studying tranquilizer,anxiolytic,and antidepressant drugs from Chinese herbs with integration for monitor,collection,analysis, storage,statistics and output of experimental data and results.更多还原