【作者】 沈佩同； 毕平； 李刚；

【Author】 Shen Peitong~1 Bi ping~(1,2) Li Gang~2 (1 tianjin medical university boimedical engineering departmeng 300070;2 tianjin university precision instrument and optoelectronics institution 300072)

【机构】 天津医科大学生物医学工程系； 天津大学精密仪器与光电子学院；

【摘要】 为了寻找一种简单可行的实验方法来降低全细胞膜片钳试验的难度,提高其工作效率,提出了采用改良的急性分离法与电流分离相结合技术,大幅度缩短了整个试验的时间,从根本上降低了膜片钳实验的难度,在全细胞电压钳制模式下,可以分别记录到电压门控钠,钾,钙通道电流.钠电流对TTX敏感,钾电流对TEA和4-AP敏感。更多还原

【Abstract】 In order to find a simple and available method to reduce the difficulties on whole-cell patch clamp experiment,improving the efficiency,a way which combines rapid dissociation and current dissociation technology is advised by this paper, shorteming the time of experimento It has reduced the difficulites of patch clamp fundametally.In the whole-cell voltage clamp mode,voltage-gated sodium,potassiu and calcium channel current can be recorded separetely.Sodium current is sensitive to TTX,potassium is sensitive to TEA and 4-AP.High-voltage activation,slow inactivation is calcium’s character.更多还原