【作者】 于杜鹃； 毛薇； 马超凡； 王亚茹； 王占彬；

【Author】 YU du-juan;MAO wei;MA chao-fan;WANG ya-ru;WANG zhan-bin;College of Animal Science and Technology,Henan University of Science and Technology;Zhengzhou Railway Vocational&Technical College;

【机构】 河南科技大学动物科技学院； 郑州铁路职业技术学院；

【摘要】 为了探讨饲粮中添加女贞子提取物对蛋鸡血液理化指标和脾脏组织结构的影响,试验选用47周龄健康海兰褐蛋鸡180只,采用单因素完全随机分组试验设计,随机分为4组,每组3个重复,每个重复15只鸡,分别为对照组(基础日粮)、试验Ⅰ组(基础日粮+0.05%女贞子提取物)、试验Ⅱ组(基础日粮+0.1%女贞子提取物)、试验Ⅲ组(基础日粮+0.15%女贞子提取物),试验期为30天。分别在试验第15、30天,每个重复随机选取3只鸡采血,测其血液理化指标。于试验第30天分别从每组随机抽取1只鸡屠宰,取其脾脏回实验室制作切片,观察其脾脏组织结构的变化。结果表明,与对照组相比,试验Ⅰ、Ⅱ组的血细胞的含量均显著提高(P<0.05),但试验Ⅲ组蛋鸡血细胞的含量显著降低(P<0.05);试验期第30天时,试验Ⅱ、Ⅲ组的总蛋白、白蛋白、球蛋白的含量显著提高(P<0.05);三个试验组蛋鸡的胆固醇、甘油三酯的含量均降低,高密度脂蛋白的含量增加,但差异不显著(P>0.05),在试验期第30天时,三个试验组蛋鸡的高密度脂蛋白的含量显著提高(P<0.05);三个试验组蛋鸡机体胆红素的含量均提高,但天门冬氨酸转氨酶的含量无太大变化(P>0.05);试验Ⅲ组脾脏淋巴细胞的数量和成熟淋巴细胞数量显著增加(P<0.05)。由此可知:在日粮中添加女贞子提取物,可以提高蛋鸡机体的免疫力,提高机体肝功能,调节其脂类的代谢。更多还原

【Abstract】 The experiment was conducted to investigate the effects of Ligustrum lucidum extract on the blood physiochemical indexes and the spleen tissue of laying hens.A total of 180 47-week-old Hy-Line Brown hens were randomly divided into 4 groups with 3 replicates per group and 15 birds per replicate in a single-factor completely randomized experimental design.The laying hens in the control group were fed a basal diet,and those in experimental groups Ⅰ,Ⅱ,Ⅲ were fed the basal diet supplemented with 0.05%,0.1%,0.15%Ligustrum lucidum extract respectively.The experiment lasted for 30 days.The blood samples of three laying hen in each replicate were collected on the 15 th and 30 th day and their blood physiochemical indexes were measured.On the 30 th day,one hen was randomly selected in each group and then killed,the spleen of which was made into slices in order to observe the changes in their spleen tissue.The results showed as follows xompared with the control group,the blood cell counts of the laying hens in experimental groups Ⅰ and Ⅱ increased significantly(P < 0.05),while that in experimental group Ⅲ decreased significantly(P < 0.05).The total protein,albumin and globin contents in the blood of laying hens in experimental group Ⅱ,insignificantly increased when it was compared with the control group(P < 0.05) on days 30.The cholesterol and triglyceride levels in the blood of laying hens in experimental groups decreased,but there was no significant difference with the control group(P > 0.05).The high-density lipoprotein levels increased significantly on the 30 th day(P < 0.05).The content of bilirubin in the blood of laying hens increased in the experimental groups,but the aspartic acid transaminase levels did not change significantly(P > 0.05).Compared with the control group,the number of lymphocytes and mature lymphocytes in the spleen increased significantly in experimental group Ⅲ(P < 0.05).In conclusion,The supplementation of Glossy Privet Fruits extracts in diets could improve the body’s immune system and the liver function of the body and regulate their lipid metabolism.更多还原