【作者】 李思明； 欧阳玲花； 唐艳强； 肖海红；

【Author】 LI Siming~(1**),OUYANG Linghua~2,TANG Yanqiang~1,XIAO Haihong~1 (1.Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science,Nanchang,Jiangxi 330200; 2.Institute of Agro-products Processing Science and Technology,Nanchang,Jiangxi 330200)

【机构】 江西省农业科学院畜牧兽医研究所； 江西省农业科学院农产品加工研究所；

【摘要】 用实时荧光定量PCR方法检测饲喂不同维生素D3剂量的丝毛乌鸡胸腺、十二指肠、空肠、回肠、盲肠和法氏囊中β-defensins 1（AvBD-1）的表达水平。结果表明,AvBD-1基因在6个组织中都有表达,其高表达水平分别在饲喂1 600 IU V_D3时的盲肠、法氏囊、空肠和胸腺,分别为饲喂800 IU V_D3时的67.239倍、18.302倍、7.367倍和6.745倍。结果提示,AvBD-1基因在同一组织的表达水平跟V_D3饲喂水平存在剂量关系,在饲喂1 600 IU时,能大幅提高AvBD-1基因在组织中的表达量。更多还原

【Abstract】 To study Taihe silky fowlβ-defensin 1（AvBD-l）gene expression level of different tissues under feeding different doses of vitamin D3.Real-time fluorescence quantitative PCR method was used to analyze the expression of AvBD-1 in thymus,duodenum,jejunum,ileum,bursa of Fabricius（BF） and cecum of Taihe silky fowl under fed different doses vitamin D3.The results showed that AvBD-1 gene expression were found in six tissues;the high levels of gene expression was in cecum,BF,jejunum and thymus at the time of feeding 1600 IU V_D3,showed by 67.239,18.302,7.367 and 6.745 times,respectively,compared with the fed 800 IU V_D3;the gene expression levels of AvBD-1 in same organizations related with the V_D3 fed dose,and the dose of feeding 1 600 IU V_D3 could greatly enhance the expression of AvBD-1 gene in tissues更多还原