【作者】 苏小艳； 胡松华； 黄一帆；

【Author】 SU Xiao-yan~1 HU Song-hua~2 HUANG Yi-fan~(1*) (1 Fujian Agriculture and Forestry University,Key Laboratory for Combination with Chinese and Western Veterinary and Animal Health of Fujian Province University,Fuzhou,350002,China 2 Zhejiang University College of Animal Sciences,Hangzhou,310058,China)

【机构】 福建农林大学中西兽医结合与动物保健福建省高校重点实验室； 浙江大学动物科学学院；

【摘要】 本研究以犬乳腺癌细胞CMT1211为模型,进行小鼠体内试验,探究EGCG的抗肿瘤作用。将CMT1211细胞培养在含10%胎牛血清的DMEM培养液中,于37℃、5%CO₂湿化的培养箱中孵育。将CMT1211以1×10⁷个/mL接种ICR小鼠,每只接种0.2mL,建立异种肿瘤种植模型,将其分为对照组、EGCG（50mg/kg）试验组。定期测量小鼠体重和肿瘤大小;采用RT-PCR检测p53、bax、bcl-2的mRNA表达量,对EGCG抗犬乳腺癌作用进行了研究,并获得如下研究结果:EGCG（50mg/kg）能有效地抑制CMT1211小鼠异位瘤体生长。肿瘤细胞内p53、bax的mRNA表达量升高,bcl-2的mRNA表达量降低。结论:EGCG可以抑制犬乳腺癌细胞CMT1211的增殖、促进细胞的凋亡。且上述作用与上调bax、p53基因,下调bcl-2基因的转录与翻译有关。更多还原

【Abstract】 This study explored the anti-tumor function of EGCG through stimulation test in vitro and mice test in vivo respectively,canine mammary cell line CMT1211 as a model.The cell line CMT1211 was cultured in Dulbecco’s Modified Eagle’s medium（DMEM）containing 10%of FBS（fetal bovine serum）,and incubated at 37℃,with 5%CO₂ and constant humidity.In vivo tests,inoculated ICR mice using CMT1211 cell to establish the transplantation tumor in ICR mice.The mice were divided into one control group EGCG（50mg/kg）and one test group.Detected the mRNA expression of p53,box,bcl-2 of mammary cancer and studied the function of EGCG anti-cancer,finally obtained the following results.The EGCG（50mg/kg）can effectively inhibit the growth of the canine mammary tumor.The mRNA expression of wild-type p53,bax increased,and bcl-2 reduced.Conclusions: EGCG had the functions of inhibiting the growth and promoting apoptosis in CMT1211 cell line.These functions were related to the up-regulating of bax,p53 and the down-regulating of bcl-2.更多还原