【作者】 冀德君； 陈仁金； 杨章平； 朱国强； 毛永江； 陈莹； 李锐；

【Author】 JI De-jun~1,CHEN Ren-jin~1,YANG Zhang-ping~1, ZHU Guo-qiang~2,MAO Yong-jiang~1,CHEN Ying~1,LI Rui~1 1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009 2.College of veterinary medicine,Yangzhou University,Yangzhou 225009

【机构】 扬州大学动物科学与技术学院； 扬州大学兽医学院；

【摘要】 [目的]趋化因子IL8是在奶牛乳腺炎发生时召集中性粒细胞而引起乳中体细胞数(SCC)急剧升高的主因子之一,了解其基因多态性将有助于对低SCC性状的选育工作。[方法]采用PCR-SSCP技术检测了来自30个公牛家系的610头中国荷斯坦牛的IL8基因5’侧翼区的多态性,并采用混合动物模型分析了多态位点与主要泌乳性状及体细胞评分(SCS)的相关性。[结果]结果显示:IL8基因5’侧翼区存在1个SNP位点,-233 A/G,定义了3种基因型,GG、GA和AA,频率分别为0.41、0.43和0.16。该SNP位点对SCS具有显著效应,对SCS指标来说,GG极显著低于GA或AA(P<0.01),而该SNP位点对测定日产奶量、测定日乳脂率、测定日乳蛋白率和305天校正产奶量影响不显著(P>0.05)。[结论]IL8基因5’侧翼区SNP-233 A/G显著影响体细胞评分。研究为进一步阐明不同奶牛乳中体细胞数变化过程的差异性机制提出了一条可行的研究路径,并可能提供有效的分子标记用于辅助低SCC性状的选育。更多还原

【Abstract】 [Objective]Bovine Interleukin 8(BL8) is one of the major factors recruiting neutrophils during cow mastitis and causing the rapid increase of somatic cell counts(SCC).Knowledge of IL8 polymorphisms would be helpful to genetic selection of low SCC.[Method]The SNPs in the 5’ upstream of bovine IL8 gene was investigated in 610 Chinese Holstein cow from 30 bull families in a dairy farm in Shanghai using Polymerase Chain Reaction-Single Strand Conformation Polymorphism(PCR-SSCP) technique,and milking traits and somatic cell score(SCS) were measured and their relationship was analyzed using a mixed animal model.[Result]The results showed that one SNP,A(-233)G,was detected, determining three genotypes,GG,GA and AA with frequencies of 0.41,0.43 and 0.16,respectively.The SNP showed significant effect on SCS,with GG having a significantly lower SCS than GA or AA(P<0.01), while this SNP had no effect on test-day milk yield,test-day milk fat content,test-day milk protein content, and 305 d corrected milk yield.[Conclusion]The findings demonstrated that the SNP-233A/G exerted significant effect on SCS and provided a relevant approach to elucidate the mechanism of the difference in cow SCC,and possible effective molecular markers for assisting the genetic selection of low SCC.更多还原